Localization of experimental calcification in rabbit blood vessels with particular reference to hemodynamics.

An observational study was made of the localization of experimental calcification of blood vessels induced by hypervitaminosis D in 15 stock rabbits, in 10 rabbits with arteriovenous fistulae, and in 6 rabbits with experimental saccular aneurysms. An arteriotomy and a phlebotomy were performed on several animals from each group. The pulmonary trunk, aorta, common carotid arteries, and external jugular veins were dissected and stained with silver nitrate to demonstrate calcification macroscopically. The first major bifurcation from 37 renal arteries from these animals was examined by the serial section technique to localize calcification about the arterial forks histologically. There was evidence that early calcification was flow related with a predilection for sites where atrophic lesions occur. Proliferative lesions in which matrix vesicles are abundant exhibited no such predisposition. It was concluded that matrix vesicles do not appear to be susceptible to vitamin D calcification. The topography of the experimental calcification differed from that of diet-induced lipid deposition.