Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA · 2003
[Role of phorbol 12-myristate 13-acetate on cytokine expression in JAR trophoblast cell line].
Аннотация на английскомСтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод пока не подготовлен
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Zhang Xi-qian, Xing Fu-qi, Li Hong, Chen Si-mei, Pang Zhan-jun, Huang Min-zhen
Аннотация
OBJECTIVE: To investigate whether phorbol 12-myristate 13-acetate (PMA) modifies the invasive ability of trophoblast cells by regulating their cytokine productions.
METHODS: Reverse transcriptase-polymerase chain reaction was used to examine the effect of PMA on the expression of cytokines which regulated the invasive ability of trophoblast cells.
RESULTS: Prior to PMA treatment, expressions of the cytokins including hepatocyte growth factor (HGF), interleukin (IL)-1beta, insulin-like growth factor (IGF)-II, transforming growth factor (TGF)- beta and vascular endothelial growth factor (VEGF) were all detected in JAR cells, only with the exception of IGF-I. After incubation with 100 nmol/L PMA for 24 h, the cells showed strong expression of IL-1beta, HGF and IGF-II, with reduced expression of TGF-beta2 and TGF-beta3.
CONCLUSION: By regulating the autocrine of these cytokines, PMA exercises its effect to enhance the invasive ability of trophoblast or choriocarcinoma cells.
METHODS: Reverse transcriptase-polymerase chain reaction was used to examine the effect of PMA on the expression of cytokines which regulated the invasive ability of trophoblast cells.
RESULTS: Prior to PMA treatment, expressions of the cytokins including hepatocyte growth factor (HGF), interleukin (IL)-1beta, insulin-like growth factor (IGF)-II, transforming growth factor (TGF)- beta and vascular endothelial growth factor (VEGF) were all detected in JAR cells, only with the exception of IGF-I. After incubation with 100 nmol/L PMA for 24 h, the cells showed strong expression of IL-1beta, HGF and IGF-II, with reduced expression of TGF-beta2 and TGF-beta3.
CONCLUSION: By regulating the autocrine of these cytokines, PMA exercises its effect to enhance the invasive ability of trophoblast or choriocarcinoma cells.
Предметные рубрики MeSH
Cell LineChoriocarcinomaCytokinesEndothelial Growth FactorsFemaleHumansIntercellular Signaling Peptides and ProteinsLymphokinesNeoplasm InvasivenessPregnancyRNA, MessengerSomatomedinsTetradecanoylphorbol AcetateTransforming Growth Factor betaTrophoblastsUterine NeoplasmsVascular Endothelial Growth Factor AVascular Endothelial Growth Factors