Fertility and sterility · 2004
Histologic and molecular changes in the ipsilateral and contralateral epididymides of the rat in response to unilateral testicular torsion followed by detorsion.
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Oh Seung-June, Kwak Cheol, Baek Minki, Kim Choung-Soo, Kim Kun Suk, Choi Hwang
Аннотация
OBJECTIVE: To investigate the histologic and molecular changes in the ipsilateral and contralateral epididymides in response to unilateral testicular torsion followed by detorsion.
DESIGN: Controlled experimental study using rats.
SETTING: Seoul National University College of Medicine, Seoul, Korea.
ANIMAL(S): Thirty-five, 8-week-old Sprague-Dawley male rats: six experimental groups and a sham-operated control group.
INTERVENTION(S): Testes of male rats were subjected to torsion for 10 minutes. At 0, 1, 4, 8, 24 hours and 1 week after detorsion, ipsilateral and contralateral epididymides were harvested. Microscopic examination and terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick-end labeling (TUNEL) staining was performed. The percentage of apoptotic ducts in the epididymis was determined by counting 100 duct cross-sections of the head and the tail of the epididymis of each specimen.
MAIN OUTCOME MEASURE(S): Morphologic change, including in situ apoptosis, of the ductal epithelium was evaluated.
RESULT(S): There was no evidence of morphologic or apoptotic changes in the 0- or 1-hour detorsion groups. However, enlargement of the interstitial fibrous tissue was found 8 and 24 hours after detorsion and marked degeneration of ductal epithelium was observed at 1 week in both the ipsilateral and contralateral epididymides. Apoptosis was first observed at 4 hours after detorsion, and the percentage of apoptotic ducts was significantly increased at 8 hours, peaked at 24 hours, and slightly decreased at 1 week in both the ipsilateral and contralateral epididymides.
CONCLUSION(S): Both morphologic changes and intracellular molecular alterations occur not only in the ipsilateral but also in the contralateral epididymis following unilateral testicular torsion/detorsion.
DESIGN: Controlled experimental study using rats.
SETTING: Seoul National University College of Medicine, Seoul, Korea.
ANIMAL(S): Thirty-five, 8-week-old Sprague-Dawley male rats: six experimental groups and a sham-operated control group.
INTERVENTION(S): Testes of male rats were subjected to torsion for 10 minutes. At 0, 1, 4, 8, 24 hours and 1 week after detorsion, ipsilateral and contralateral epididymides were harvested. Microscopic examination and terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick-end labeling (TUNEL) staining was performed. The percentage of apoptotic ducts in the epididymis was determined by counting 100 duct cross-sections of the head and the tail of the epididymis of each specimen.
MAIN OUTCOME MEASURE(S): Morphologic change, including in situ apoptosis, of the ductal epithelium was evaluated.
RESULT(S): There was no evidence of morphologic or apoptotic changes in the 0- or 1-hour detorsion groups. However, enlargement of the interstitial fibrous tissue was found 8 and 24 hours after detorsion and marked degeneration of ductal epithelium was observed at 1 week in both the ipsilateral and contralateral epididymides. Apoptosis was first observed at 4 hours after detorsion, and the percentage of apoptotic ducts was significantly increased at 8 hours, peaked at 24 hours, and slightly decreased at 1 week in both the ipsilateral and contralateral epididymides.
CONCLUSION(S): Both morphologic changes and intracellular molecular alterations occur not only in the ipsilateral but also in the contralateral epididymis following unilateral testicular torsion/detorsion.