[Helicobacter pylori in mucous membrane of stomach and duodenum of patients with symptoms of ulceration and dyspepsia].

The material consisted of samples of mucous membrane of stomach and duodenum obtained during endoscopy in patients with clinical symptoms of peptic ulcer of stomach or the duodenum or with dyspeptic problems. Samples were tested for presence of H. pylori by culture on brain-heart agar supplemented with 7% of horse blood. Direct test for urease production was also performed. Isolated strains were identified basing on morphology of growth, Gram-stained preparation, mobility of microorganism, production of oxidase, catalase and urease, and ability to agglutinate in immune goat serum for standard H. pylori strain. Out of tested 217 samples, positive result was obtained in 141 cases. Urease test was positive in 138 cases. Isolated strains were tested for susceptibility to 14 antimicrobials. They were all resistant to nalidixic acid and susceptible in 90-100% to cephalothin, furazolidone, gentamicin and ofloxacin.