Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi · 2001
[A preliminary study on translocation of the immunoglobulin heavy chain gene in KM3 myeloma cell line].
Аннотация на английскомСтатьяНазвание и аннотация: Перевод пока не подготовлен
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Shen H, Hou J, Liu H
Аннотация
OBJECTIVE: To identify the IgH translocation in KM3 myeloma cell line established from a Chinese patients.
METHODS: Southern blotting was performed with dig-labeled probes specific for IgH switch regions to identify and distinguish IgH switch recombination events.
RESULTS: Besides a 10.0 kb Hind III fragment being able to hybridize with both 5' S mu and 3' S mu probes as that in placenta genome DNA. There was a 5.6 kb Hind III fragment in KM3 genome DNA could only hybridize with the 3' S mu probe.
CONCLUSION: There was an illegitimate switch recombination fragment containing 3' S mu region in the KM3 myeloma cell line, which was caused by the IgH translocation involving the IgH S mu region.
METHODS: Southern blotting was performed with dig-labeled probes specific for IgH switch regions to identify and distinguish IgH switch recombination events.
RESULTS: Besides a 10.0 kb Hind III fragment being able to hybridize with both 5' S mu and 3' S mu probes as that in placenta genome DNA. There was a 5.6 kb Hind III fragment in KM3 genome DNA could only hybridize with the 3' S mu probe.
CONCLUSION: There was an illegitimate switch recombination fragment containing 3' S mu region in the KM3 myeloma cell line, which was caused by the IgH translocation involving the IgH S mu region.