[A preliminary study on translocation of the immunoglobulin heavy chain gene in KM3 myeloma cell line].

OBJECTIVE

To identify the IgH translocation in KM3 myeloma cell line established from a Chinese patients.

METHODS

Southern blotting was performed with dig-labeled probes specific for IgH switch regions to identify and distinguish IgH switch recombination events.

RESULTS

Besides a 10.0 kb Hind III fragment being able to hybridize with both 5' S mu and 3' S mu probes as that in placenta genome DNA. There was a 5.6 kb Hind III fragment in KM3 genome DNA could only hybridize with the 3' S mu probe.

CONCLUSION

There was an illegitimate switch recombination fragment containing 3' S mu region in the KM3 myeloma cell line, which was caused by the IgH translocation involving the IgH S mu region.