Au-doped MoS2 nanozyme with self-cascade catalytic performance for one-step glucose detection application.

Glucose oxidase (GOx), which specifically catalyzes glucose oxidation, has been widely employed in constructing various biosensors for clinical screening of diseases such as diabetes and tumors. However, current GOx-based glucose detection methods suffer from limitations including high costs and tedious multi-step procedures. In this work, we developed a facile green aqueous-phase synthesis of Au-doped MoS2 nanomaterials with dual-enzyme activities. The planar structure of MoS2 serves as an excellent support for Au deposition, ensuring good dispersion stability. Moreover, Au doping endows the material with GOx-like activity, while the hybrid nanostructure exhibits enhanced peroxidase-like activity due to interfacial interactions in the bimetallic system. The as-prepared Au-MoS2 nanozyme can trigger a self-cascading reaction, enabling one-step colorimetric glucose detection with a wide linear range and an ultralow detection limit of 0.09 mM. More importantly, practical tests using biological samples (including hepatocellular carcinoma cells and diabetic urine samples) demonstrate the excellent potential for real-world applications. This newly developed material significantly reduces both the time and cost of glucose detection while maintaining good stability and reproducibility, showing promising prospects for clinical diagnostics.