Single-atom aptamer anchoring enables light-addressable multiplexed biosensing for early detection of pancreatic cancer.

Pancreatic cancer remains one of the deadliest malignancies due to its silent progression and lack of early diagnostic tools. Here, we report a light-addressable photoelectrochemical (LAMT-PEC) biosensing system featuring single-atom Au1-TiO2 photoelectrodes for the simultaneous detection of five pancreatic cancer biomarkers in blood: CA19-9, CEA, AFP, CA125, and CA724. By anchoring aptamers at atomic precision, the platform achieves sub-pg/mL sensitivity, minimizes nonspecific binding, and demonstrates high anti-interference capability. When coupled with a machine learning algorithm, the system enables precise discrimination of early-stage pancreatic cancer from healthy individuals with an AUC of 97.4 %, significantly outperforming CA19-9 alone. The LAMT-PEC platform presents a scalable, high-throughput, and clinically relevant strategy for early cancer screening, with broad applicability to multiplex diagnostics.