Shanghai kou qiang yi xue = Shanghai journal of stomatology · 2009
[The effect of VEGF-ASODN transfection on expression of VEGF and growth in tongue squamous cancer cell line Tca8113].
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Li Xiao-guang, Wang Yan-xiu, Zhou Bao-qin, Qi Chun-hua
Аннотация
PURPOSE: To evaluate the effect of transfection of vascular endothelial growth factor antisense oligonucleotide (VEGF-ASODN) on expression of vascular endothelial growth factor(VEGF) and growth in tongue cancer cells.
METHODS: The VEGF-ASODN was synthesized with phosphorothioic acid. After transfecting with VEGF-ASODN in tongue cancer cells Tca8113, the quantity of VEGFmRNA and protein was detected by hybridization in situ and immunocytochemistry, and the quantity of VEGF protein in supernatant was detected by ELISA. FCM and MTT methods were used to detect cellular apoptosis and the activity of cells, respectively. The effect of transfection on the growth of cells was evaluated by growth curve. SPSS12.0 software package was used for statistical analysis.
RESULTS: The quantity of VEGF mRNA, protein levels of VEGF in the cells and in culture fluid decreased after transfection of VEGF-ASODN (P<0.01,P<0.05).The ratio of apoptosis increased, the activity of cells also decreased after transfection VEGF-ASODN (P<0.01).
CONCLUSIONS: Transfection with VEGF-ASODN in Tca8113 cells can inhibit the expression of VEGF remarkably. It can enhance cellular apoptosis and suppress growth of cells. Supported by Research Fund from Science and Technology Bureau of Taian City (Grant No.2007-46).
METHODS: The VEGF-ASODN was synthesized with phosphorothioic acid. After transfecting with VEGF-ASODN in tongue cancer cells Tca8113, the quantity of VEGFmRNA and protein was detected by hybridization in situ and immunocytochemistry, and the quantity of VEGF protein in supernatant was detected by ELISA. FCM and MTT methods were used to detect cellular apoptosis and the activity of cells, respectively. The effect of transfection on the growth of cells was evaluated by growth curve. SPSS12.0 software package was used for statistical analysis.
RESULTS: The quantity of VEGF mRNA, protein levels of VEGF in the cells and in culture fluid decreased after transfection of VEGF-ASODN (P<0.01,P<0.05).The ratio of apoptosis increased, the activity of cells also decreased after transfection VEGF-ASODN (P<0.01).
CONCLUSIONS: Transfection with VEGF-ASODN in Tca8113 cells can inhibit the expression of VEGF remarkably. It can enhance cellular apoptosis and suppress growth of cells. Supported by Research Fund from Science and Technology Bureau of Taian City (Grant No.2007-46).