Experimental cell research · 2025
lncRNA NR_146969 promotes the progression of lung adenocarcinoma.
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Chen Ying, Wang Juan, Gu Linguo, Chen Hongzuo, Gai Zhengling, Hu Rui, Qing Bei, Yuan Yunchang, Xia Zhenkun
Аннотация
BACKGROUND: Emerging research suggests that dysregulation of long non-coding RNAs (lncRNAs) is closely linked to the onset and progression of cancer. In this study, we used lncRNA array technology to identify differentially expressed lncRNAs in lung adenocarcinoma patients and normal lung tissues. The study further explored the clinical significance and function of candidate lncRNAs in lung adenocarcinoma (LUAD). The results showed that lncRNA NR_146969 was upregulated in LAUD specimens and was associated with lymph node metastasis and clinical staging in LUAD patients.
METHODS: The biological functions of lncRNA NR_146969 were observed using CCK-8, colony formation, transwell assay and xenograft tumor model. Explore the potential mechanism of action of lncRNA NR_146969 by FISH, dual luciferase reporter assay and recovery assay.
RESULTS: Overall, lncRNA NR_146969 plays an oncogenic role in LUAD. Mechanically, lncRNA NR_146969 targets SLC6A14 via miR-26a-1-3p, leading to phosphorylation of the AKT/mTOR pathway, which promotes LUAD growth and metastasis.
CONCLUSION: Therefore, targeting lncRNA NR_146969 may provide a new therapeutic strategy for LUAD.
METHODS: The biological functions of lncRNA NR_146969 were observed using CCK-8, colony formation, transwell assay and xenograft tumor model. Explore the potential mechanism of action of lncRNA NR_146969 by FISH, dual luciferase reporter assay and recovery assay.
RESULTS: Overall, lncRNA NR_146969 plays an oncogenic role in LUAD. Mechanically, lncRNA NR_146969 targets SLC6A14 via miR-26a-1-3p, leading to phosphorylation of the AKT/mTOR pathway, which promotes LUAD growth and metastasis.
CONCLUSION: Therefore, targeting lncRNA NR_146969 may provide a new therapeutic strategy for LUAD.
Предметные рубрики MeSH
RNA, Long NoncodingHumansAdenocarcinoma of LungLung NeoplasmsAnimalsGene Expression Regulation, NeoplasticDisease ProgressionCell ProliferationMiceMicroRNAsFemaleMaleCell MovementMice, NudeCell Line, TumorMiddle AgedTOR Serine-Threonine KinasesMice, Inbred BALB CLymphatic MetastasisSignal TransductionProto-Oncogene Proteins c-akt