Expression profiles of the sirtuin genes family during ovarian development and hormonal regulation in Pampus argenteus.

Sirtuins (Sirt) are a class of NAD + -dependent type III histone deacetylases that play significant roles in energy metabolism and reproductive regulation. To explore the role of the sirt genes family in the ovarian development of Pampus argenteus and its significance in artificial breeding, this study conducted a systematic investigation using phylogenetic tree analysis and real-time fluorescence quantitative PCR. Phylogenetic analysis indicated that the sirt gene of the P. argenteus was most closely related to those of Thunnus maccoyii and Thunnus. albacares. Specific expression analysis revealed that the expression levels of sirt1, sirt2, sirt5, sirt6, and sirt7 in the ovary were significantly higher than those in other tissues (P < 0.05). In the analysis of ovarian development during stage I-VI, sirt1, sirt3, and sirt5 reached their peak expression during the pre-maturation stage, while sirt6 had the highest expression in stage VI. The in vitro steroid hormone immature egg culture experiment showed that estradiol significantly inhibited the expression of sirt1, sirt2, sirt4, sirt5, sirt6 genes. Luteinizing hormone inhibited the expression of sirt1, sirt2 and sirt4, but promoted the expression of sirt3 and sirt5. Human chorionic gonadotropin slightly upregulated the expression of sirt1. Intraperitoneal injection of HCG and LH shows differential regulation on the expression of sirt genes in different brain regions, indicating that they were involved in the neuroendocrine regulatory pathway. In conclusion, the sirt genes family, especially sirt1, which showed peak expression during the critical period of early egg growth and before maturation, may promote ovarian maturation by regulating meiotic recovery and energy metabolism pathways. This study provides a theoretical basis for researching the reproductive regulation of P. argenteus and optimizing artificial breeding techniques.