Journal of assisted reproduction and genetics · 2026
Ovary laparoscopic incision and intraovarian platelet-rich plasma induce distinct cortical stiffness changes and follicular activation in aged sheep-a pilot study.
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Justo Marta Méndez, Dos Anjos Janisse Ferreri, Herranz-Diez Carolina, Diaz Jordi Otero, Arroyo Jose Antonio Domínguez, Miguel Ignacio Santiago Álvarez, Mateos Soledad Sanchez, Calafell Josep Maria, Vallejos Salvadora Civico, Parraga Ester, Farre Ramon, Gargallo Francisco Miguel Sanchez, Gasol Francesc Fabregues
Аннотация
PURPOSE: Poor ovarian response and primary ovarian insufficiency remain major challenges in assisted reproductive technologies. Recent advances propose mechanical approaches-such as in vitro activation, drug-free IVA, and whole ovarian laparoscopic incision (WOLI)-as well as biological strategies like platelet-rich plasma (PRP) injections to improve ovarian function. This study aims to evaluate the impact of WOLI and PRP on ovarian follicular activation, with a particular focus on the associated biomechanical alterations in the ovarian cortex.
METHODS: Experimental pilot study includes six aged female Merino sheep. Group A underwent WOLI on one ovary, and group B received PRP injections. Contralateral ovaries served as controls. Laparoscopic bilateral oophorectomies were performed at 2, 4, and 6 months. Atomic force microscopy (AFM) measured ovarian cortical stiffness, and histological analysis assessed follicular activation. Analyses were performed by comparing the operated ovary to the contralateral, non-operated ovary.
RESULTS: AFM measurements showed that in group A, ovarian stiffness significantly increased at 6 months (8.683 kPa vs. 5.551 kPa; p < 0.001), with no significant differences at 2 or 4 months. In group B, stiffness increased significantly at 2 months (11.177 kPa vs. 6.590 kPa; p < 0.001), but not at 4 or 6 months post-intervention. Growing follicle proportions differed between treated and control ovaries respectively, with activation at 2 months (58.95% vs. 29.73%) in group A and 6 months in group B (53.01% vs. 20.33%).
CONCLUSION: The study suggests that the activating effect on ovarian folliculogenesis of both the surgical procedure and PRP administration shows a different time course and that could be related to its effect on ovarian cortex mechanobiology.
METHODS: Experimental pilot study includes six aged female Merino sheep. Group A underwent WOLI on one ovary, and group B received PRP injections. Contralateral ovaries served as controls. Laparoscopic bilateral oophorectomies were performed at 2, 4, and 6 months. Atomic force microscopy (AFM) measured ovarian cortical stiffness, and histological analysis assessed follicular activation. Analyses were performed by comparing the operated ovary to the contralateral, non-operated ovary.
RESULTS: AFM measurements showed that in group A, ovarian stiffness significantly increased at 6 months (8.683 kPa vs. 5.551 kPa; p < 0.001), with no significant differences at 2 or 4 months. In group B, stiffness increased significantly at 2 months (11.177 kPa vs. 6.590 kPa; p < 0.001), but not at 4 or 6 months post-intervention. Growing follicle proportions differed between treated and control ovaries respectively, with activation at 2 months (58.95% vs. 29.73%) in group A and 6 months in group B (53.01% vs. 20.33%).
CONCLUSION: The study suggests that the activating effect on ovarian folliculogenesis of both the surgical procedure and PRP administration shows a different time course and that could be related to its effect on ovarian cortex mechanobiology.