Selenium and mercury tissue partitioning and trophodynamics in the Lake Koocanusa (USA-Canada) fish community.

Mining-related contaminants such as selenium (Se) and mercury (Hg) accumulate in aquatic organisms and transfer through aquatic food webs, where they can exert toxic effect undermining the ecological health of aquatic resources. Yet, how Se and Hg co-distribute within food webs and within individual organisms remains poorly understood. We compiled muscle and ovary Se and Hg concentration data from fishes captured in Lake Koocanusa-a North American reservoir receiving elevated Se and Hg inputs from coal mining operations in the Elk River Valley, Canada-to provide insights into the co-processing of Se and Hg within fish and among fish species, including how ecological or seasonal factors shift these distribution patterns. Se and Hg concentrations (ranges: 0.21-38.7 μg Se g-1 dry weight and 0.01-0.84 μg Hg g-1 wet weight), as well as Se:Hg molar ratios, varied substantially between tissues and among species, reflecting biological and ecological differences. Our results suggest that the decoupling of Se or Hg deposition in fish muscle versus ovary may reflect different pathways of impairment, at the individual and population levels, and that interspecific and tissue-specific variability makes it challenging to set a universal Se:Hg threshold for the protection of aquatic organisms.