Placenta · 2025
Hystero-embryoscopy as a tool for RPL immunological research at the maternal-fetal interface.
СтатьяМногоцентровое исследованиеНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Bruno Valentina, Zizolfi Brunella, Ferretti Margherita, Di Martino Simona, Arteni Brindusa Ana Maria, Betti Martina, Ciuffreda Ludovica, De Nicola Francesca, Scalera Stefano, Cariati Federica, De Angelis Chiara, Nardelli Fabiola, Giudice Matteo, Mancini Emanuela, Baiocco Ermelinda, Russo Mario, Pallocca Matteo, Fanciulli Maurizio, Piaggio Giulia, Carosi Mariantonia, Di Spiezio Sardo Attilio, Vizza Enrico
Аннотация
INTRODUCTION: An abnormal immune response at the fetal-maternal interface is expected in 50-60 % of unexplained Recurrent Pregnancy Loss (uRPL) cases. Detected immunophenotypes in uRPL could help in risk assessment, prognosis and therapy. The study of immune mechanisms at the fetal-maternal interface is crucial, but the technique used for research has limitations, including contamination and invasiveness, which can trigger immune responses. Hystero-embryoscopy may provide a precise method for studying the immunological factors involved in RPL at the maternal-fetal interface, reducing bias from sample collection techniques. Our aim is to assess its effectiveness in obtaining high-quality samples to study the immunological basis of RPL.
METHODS: This is a multicenter prospective study in which 10 women with a first-trimester ongoing miscarriage were enrolled and received surgical treatment at the University Hospital Federico II in Naples. Embryo-hysteroscopy was used to selectively obtain decidual and chorionic villous tissues separately, from the maternal-fetal interface. Transcriptome sequencing was performed at Regina Elena National Cancer Institute in Rome.
RESULTS: RNA integrity numbers (RIN) satisfied the minimum quality requirements (median RIN considering all samples was 8.4 ± 1.1) and RNA sequencing exhibited adequate sequencing depth (the mean of Uniquely Mapped Reads considering all samples was 52.1 ± 7.7), ensuring reliable downstream analysis. The bioinformatics analysis demonstrated the absence of cross-tissue contamination due to the clear separation between the transcriptional profiles of decidual and chorionic villous tissues: CD45 immunostaining and pathological validation confirmed these findings.
CONCLUSIONS: These findings demonstrate that hystero-embryoscopy enables precise immunological profiling at the maternal-fetal interface while minimizing sample contamination.
METHODS: This is a multicenter prospective study in which 10 women with a first-trimester ongoing miscarriage were enrolled and received surgical treatment at the University Hospital Federico II in Naples. Embryo-hysteroscopy was used to selectively obtain decidual and chorionic villous tissues separately, from the maternal-fetal interface. Transcriptome sequencing was performed at Regina Elena National Cancer Institute in Rome.
RESULTS: RNA integrity numbers (RIN) satisfied the minimum quality requirements (median RIN considering all samples was 8.4 ± 1.1) and RNA sequencing exhibited adequate sequencing depth (the mean of Uniquely Mapped Reads considering all samples was 52.1 ± 7.7), ensuring reliable downstream analysis. The bioinformatics analysis demonstrated the absence of cross-tissue contamination due to the clear separation between the transcriptional profiles of decidual and chorionic villous tissues: CD45 immunostaining and pathological validation confirmed these findings.
CONCLUSIONS: These findings demonstrate that hystero-embryoscopy enables precise immunological profiling at the maternal-fetal interface while minimizing sample contamination.