Wei sheng yan jiu = Journal of hygiene research · 2015
[Effects of bisphenol A on voltage-dependent T-type calcium channels in mouse testis and epididymis, and the role of estrogen receptors].
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Wang Qiang, Zhang Ling, Ding Zeyuan, Qian Wenyi, Lu Qian, Wang Jun, Xiao Hang
Аннотация
OBJECTIVE: To explore the possible effects of bisphenol A (BPA) on voltage-dependent T-type calcium channels in mouse testis and epididymis, the role of the two ERs was also investigated.
METHODS: Adult male wild type (WT), ERs knock out mice (αERKO and βERKO) were randomly divided into control and BPA [100 μg/(kg. d) for 30 days via intragastric administration] treatment groups. Quantitative real time PCR was used to examine Cav3.1, Cav3.2 and Cav3.3 genes of the testis and epididymis.
RESULTS: In the testis, the mRNA expression of Cav3.1, Cav3.2 and Cav3.3 were increased in BPA-treated animals. In the epididymis, the mRNA expression of Cav3. 1 and Cav3.2 were increased, however, Cav3.3 were decreased in BPA-treated animals. BPA up-regulation of Cav3.1 and Cav3.2 mRNA expression of the epididymis were dependent on both ERα and ERβ, whereas BPA regulation of Cav 3.1 - Cav3.3 of the testis and Cav3.3 of the epididymis appeared to be dependent on ERβ only.
CONCLUSION: It suggested that the ERs signalling was necessary for the regulation of the Cav3 channels mRNA expression by BPA, which could affect the male reproductive function.
METHODS: Adult male wild type (WT), ERs knock out mice (αERKO and βERKO) were randomly divided into control and BPA [100 μg/(kg. d) for 30 days via intragastric administration] treatment groups. Quantitative real time PCR was used to examine Cav3.1, Cav3.2 and Cav3.3 genes of the testis and epididymis.
RESULTS: In the testis, the mRNA expression of Cav3.1, Cav3.2 and Cav3.3 were increased in BPA-treated animals. In the epididymis, the mRNA expression of Cav3. 1 and Cav3.2 were increased, however, Cav3.3 were decreased in BPA-treated animals. BPA up-regulation of Cav3.1 and Cav3.2 mRNA expression of the epididymis were dependent on both ERα and ERβ, whereas BPA regulation of Cav 3.1 - Cav3.3 of the testis and Cav3.3 of the epididymis appeared to be dependent on ERβ only.
CONCLUSION: It suggested that the ERs signalling was necessary for the regulation of the Cav3 channels mRNA expression by BPA, which could affect the male reproductive function.