Performance Evaluation of a New Generation of Automated Analyzer for Pleural and Peritoneal Fluids Cytology.

BACKGROUND

Hematologic counters with dedicated body fluids mode allows these samples to be analyzed in an automated manner. Our aim was to evaluate the performance of the Sysmex XN-3000 and compare it with the Sysmex XE-5000, in use in our laboratory for cytological analysis of body fluids.

METHODS

We studied 108 pleural and peritoneal fluids. Laboratory routine included manual and automated cell counts. The Sysmex XN-3000 validation protocol included precision, carryover, linearity studies, and comparison with traditional microscopic differential counts and with the analyzer in use.

RESULTS

Sysmex XN-3000 met all the criteria for analytical quality with strong correlation with microscopy (r = 0.95 for MN and PMN) and agreement of 93% (kappa = 0.813, p < 0.0001). Comparison between both analyzers revealed no significant differences and strong correlation regarding WBC and RBC (r > 0.98), mononuclear and polymorphonuclear cells (r = 0.99).

CONCLUSIONS

Sysmex XN-3000 showed strong correlation and agreement with traditional microscopy with an equivalent performance compared to the XE-5000.