Archives of oral biology · 2010
Specific expression of salivary maxi-K channel variant is augmented in diabetic mice.
СтатьяНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Okamura Kazuhiko, Kato Kenichi, Uchida Ryuji, Ohkubo Tsuyako, Taniguchi Kunihisa, Yamazaki Jun
Аннотация
BACKGROUND AND OBJECTIVE: A genetically diabetic mouse strain (db/db) exhibits severe obesity and a syndrome resembling human non-insulin-dependent diabetes mellitus. Our histological study of submandibular glands revealed that the size and area of the granular convoluted tubules was substantially decreased in db/db mice. We hypothesized that this structural difference reflected a specific alteration in salivary duct function.
METHODS: The saliva evoked by pilocarpine was used for the measurement of ion concentrations, and submandibular glands were dissected out for the immunohistochemistry and real-time PCR study.
RESULTS: The K(+) concentration of the salivary secretion was higher in db/db than in control m+/m+ mice, while neither saliva volume nor the concentrations of Na(+) or Cl⁻ differed between these strains. In db/db mice (vs. m+/m+ mice): quantitative PCR analysis revealed an increased mRNA expression of large-conductance Ca²(+)-activated K(+) (maxi-K) channels, immunohistochemistry revealed an increase in the luminal surface expression of the maxi-K channel protein, and a particularly interesting finding was that there was a substantial increase in the salivary tissue-specific splice variant ParSlo.
CONCLUSION: These results suggest that in db/db mice, the K(+) content of saliva may be elevated due to an expression of a maxi-K channel variant, which results from a modification of ductal structure.
GENERAL SIGNIFICANCE: Our data may shed some light on the mechanism responsible for determining the dynamics of salivary K(+) concentration increased in diabetic patients.
METHODS: The saliva evoked by pilocarpine was used for the measurement of ion concentrations, and submandibular glands were dissected out for the immunohistochemistry and real-time PCR study.
RESULTS: The K(+) concentration of the salivary secretion was higher in db/db than in control m+/m+ mice, while neither saliva volume nor the concentrations of Na(+) or Cl⁻ differed between these strains. In db/db mice (vs. m+/m+ mice): quantitative PCR analysis revealed an increased mRNA expression of large-conductance Ca²(+)-activated K(+) (maxi-K) channels, immunohistochemistry revealed an increase in the luminal surface expression of the maxi-K channel protein, and a particularly interesting finding was that there was a substantial increase in the salivary tissue-specific splice variant ParSlo.
CONCLUSION: These results suggest that in db/db mice, the K(+) content of saliva may be elevated due to an expression of a maxi-K channel variant, which results from a modification of ductal structure.
GENERAL SIGNIFICANCE: Our data may shed some light on the mechanism responsible for determining the dynamics of salivary K(+) concentration increased in diabetic patients.