The effect of five days calcium chloride treatment on the somatostatin cells of the antral mucosa in white rats.

The effects of a five day treatment with calcium chloride (1 ml 0.11 M CaCl2/100g body weight i.p., twice daily) on somatostatin cells in gastric antral mucosa of white rats were investigated. Somatostatin cells of experimental and control animals were identified by immunoperoxidase method using rabbit antihuman antisomatostatin (1:1000), and counted on perpendicularly cut sections taken from four transversal levels going from pylorus to antrofundic border. The results obtained have demonstrated the occurrence of somatostatin cell hyperplasia in animals treated with calcium chloride, established on the ground of highly significant rise in the number of somatostatin cells and a change in their topography.