In ovo feeding of creatine monohydrate increases performances of hatching and development in breeder chicks.

The current investigation was conducted to test the potential effects of in ovo feeding of creatine monohydrate (CMH) on hatchability, embryonic mortality, hatching weight, and development of heart and gastrointestinal tract (main organs and regions) of breeder chick embryos. Rhode Island Red fertile eggs were randomly distributed into seven experimental treatments: untreated egg (control), a sterile buffered solution (0.50% NaCl), and five solutions containing increased levels of CMH (0.50, 1.00, 1.50, and 2.00%) + 0.50% NaCl, being separated in four groups/replicates (three with 15 eggs and one with 16 eggs), totaling 61 eggs/treatment and a total of 427 fertile eggs used. All-in ovo injected groups with CMH decreased the hatchability and increased the intermediary embryonic mortality. At hatching, all-in ovo injected groups with CMH also increased the hatching weight and stimulated the development of the heart and the total length of the gastrointestinal tract, especially important organs for digestion of nutrients (yolk sac, pro-ventricle and gizzard) and regions for nutrient absorption (jejunum + ileum and colon + rectum). Conclusively, the in ovo feeding using CMH showed positive impacts on hatching weight and the development of gastrointestinal tract of chicks. However, caused negative impacts on hatchability.