Alterations in the Gut Microbiota and Metabolite Profiles of Lung Cancer Patients.

Lung cancer is the leading cause of cancer-related deaths worldwide. The gut microbiota is known to be involved in the occurrence and development of a variety of cancers, but the role of gut microbiota in lung cancer remains unclear. Here, we selected fecal samples of lung cancer patients and healthy people for 16S rRNA high-throughput sequencing and untargeted metabolomics analysis, respectively. The results showed that Actinobacteria, Proteobacteria, Patescibacteria, Cyanobacteria and Verrucomicrobiota were enriched in the LC; And the interaction between bacteria in the LC was more complicated than that in the NC. ROC showed that Actinobacteriota, Bacteroidota, Verrucomicrobiota and 7 kinds of bacteria at the genus have a better resolution for LC There are 82 kinds of metabolites that were different between NC and LC, mainly lipid metabolism molecules KEGG analysis indicated that Glycerophospholipid metabolism and alpha-Linolenic acid metabolism may be different in the LC and NC. ROC showed that 15 kinds of metabolites had a better classification effect on LC and NC; And we found that the correlation between metabolites in NC was higher than that in LC. This article pointed out the direction for follow-up research on its role in the occurrence and development of lung cancer.