Cellular oncology : the official journal of the International Society for Cellular Oncology · 2006
The CT-element of the c-myc gene does not predispose to chromosomal breakpoints in Burkitt's lymphoma.
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Weber Achim, Gutierrez Marina I, Levens David
Аннотация
BACKGROUND: Chromosomal translocations are causally related to the development of many tumors. In Burkitt's lymphoma, abnormalities involving the c-myc gene are essential. The CT-element of the c-myc promoter adopts non-B-conformation in vivo and in vitro, and therefore provides a potential fragile site.
METHODS: We have developed a LM-PCR-based approach to test if chromosomal breakpoints indeed cluster in this region.
RESULTS: Amplifying both, wild-type as well as the translocated c-myc gene by LM-PCR, it was shown that chromosomal breakpoints did not cluster within the CT-element.
CONCLUSIONS: Therefore, the CT-element is not especially susceptible to the formation of breakpoints leading to chromosomal translocations in Burkitt's lymphoma.
METHODS: We have developed a LM-PCR-based approach to test if chromosomal breakpoints indeed cluster in this region.
RESULTS: Amplifying both, wild-type as well as the translocated c-myc gene by LM-PCR, it was shown that chromosomal breakpoints did not cluster within the CT-element.
CONCLUSIONS: Therefore, the CT-element is not especially susceptible to the formation of breakpoints leading to chromosomal translocations in Burkitt's lymphoma.