Auris, nasus, larynx · 2022
Hsa_circ_0081534 facilitates malignant phenotypes by sequestering miR-874-3p and upregulating FMNL3 in nasopharyngeal carcinoma.
СтатьяНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
He Jintian, Chen Shunjin, Wu Xiaoying, Jiang Di, Li Runqiu, Mao Zhiqiang
Аннотация
OBJECTIVE: Circular RNAs (circRNAs) are connected to nasopharyngeal carcinoma (NPC) development and progression. CircRNA hsa_circ_0081534 (circ_0081534) has been reported to be associated with NPC progression, but its underlying regulatory mechanisms are largely unknown. Thus, the study aims to investigate the mechanism by which circ_0081534 regulates NPC progression.
METHODS: Quantitative reverse transcription polymerase chain reaction was conducted to detect circ_0081534 expression. Loss-of-function assays were conducted to evaluate the role of circ_0081534, including 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT), 5-ethynyl-2'-deoxyuridine (EdU) staining, colony formation, flow cytometry, transwell, western blotting, and xenograft assays. Targeting relationship was identified through dual-luciferase reporter assay and RNA immunoprecipitation.
RESULTS: Our data exhibited that circ_0081534 was upregulated in NPC samples and cells. Knockdown of circ_0081534 repressed NPC cell proliferation, migration, invasion, EMT, and triggered NPC cell apoptosis. Also, circ_0081534 silencing decreased NPC cell growth in xenograft models. Circ_0081534 functioned as a miR-874-3p sponge, and downregulation of miR-874-3p alleviated the suppressive effects of circ_0081534 silencing on NPC cell malignant phenotypes. MiR-874-3p targeted FMNL3, and circ_0081534 regulated FMNL3 expression through serving as a miR-874-3p sponge. Upregulation of FMNL3 relieved the inhibitory effects of circ_0081534 downregulation on NPC cell malignant phenotypes.
CONCLUSION: circ_0081534 interference repressed NPC progression partly by modulating the miR-874-3p/FMNL3 axis.
METHODS: Quantitative reverse transcription polymerase chain reaction was conducted to detect circ_0081534 expression. Loss-of-function assays were conducted to evaluate the role of circ_0081534, including 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT), 5-ethynyl-2'-deoxyuridine (EdU) staining, colony formation, flow cytometry, transwell, western blotting, and xenograft assays. Targeting relationship was identified through dual-luciferase reporter assay and RNA immunoprecipitation.
RESULTS: Our data exhibited that circ_0081534 was upregulated in NPC samples and cells. Knockdown of circ_0081534 repressed NPC cell proliferation, migration, invasion, EMT, and triggered NPC cell apoptosis. Also, circ_0081534 silencing decreased NPC cell growth in xenograft models. Circ_0081534 functioned as a miR-874-3p sponge, and downregulation of miR-874-3p alleviated the suppressive effects of circ_0081534 silencing on NPC cell malignant phenotypes. MiR-874-3p targeted FMNL3, and circ_0081534 regulated FMNL3 expression through serving as a miR-874-3p sponge. Upregulation of FMNL3 relieved the inhibitory effects of circ_0081534 downregulation on NPC cell malignant phenotypes.
CONCLUSION: circ_0081534 interference repressed NPC progression partly by modulating the miR-874-3p/FMNL3 axis.