MMP2 rs243865 and MMP8 rs11225395 Gene Polymorphisms Are Correlated With Breast Cancer Risk in Bangladeshi Women: Evidence From a Case-Control Study.

BACKGROUND

Variants of the matrix metalloproteinase (MMP) gene have been associated with multiple malignancies, including stomach, bladder, lung, breast, melanoma, squamous cell skin, and colorectal cancers. Genetic factors often slightly to moderately increase the risk of breast cancer.

OBJECTIVE

This study was conducted to ascertain the correlation between breast cancer patients in Bangladesh and the MMP2 rs243865 and MMP8 rs11225395 polymorphisms.

DESIGN

A case-control study.

METHODS

The tetra-primer amplification refractory mutation system polymerase chain reaction (ARMS-PCR) was used for genotyping of 324 healthy individuals and 315 individuals with breast cancer. In addition, we performed in silico expression analysis of MMP2 and MMP8 genes using different databases, such as GEPIA, UALCAN, and GTEx.

RESULTS

In the case of MMP2 rs243865 polymorphism, additive model 1 (OR = 1.90, 95% CI = 1.03-3.52), dominant model (OR = 2.07, 95% CI = 1.15-3.72), overdominant model (OR = 1.83, 95% CI = 0.99-3.38), and allelic (OR = 1.93, 95% CI = 1.17-3.17) model were significantly associated with enhanced breast cancer risk. For the MMP8 rs11225395 polymorphism, none of the genetic models showed a significant association with increased breast cancer risk. The logistic regression analyses were adjusted for response status (age, BMI, and marital status). In silico analysis showed that MMP2 was more highly expressed in normal tissues, whereas MMP8 was more highly expressed in breast tissues.

CONCLUSION

Our findings imply that MMP2 (rs243865) polymorphisms correlate with higher breast carcinoma risk but have no relationship with MMP8 (rs11225395) polymorphisms in the Bangladeshi female population.