European journal of gynaecological oncology · 2017
Inhibitory effects of miR-101 overexpression on cervical cancer SiHa cells.
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Liu H, Zhao J, Lv J
Аннотация
PURPOSE OF INVESTIGATION: microRNAs (miRNAs), which can regulate cell biological behaviors such as proliferation and apoptosis as oncogenes or anti-oncogenes, are closely associated with cancer onset and progression. The aim of this study was to detect the expres- sion changes of miR-101 in cervical cancer tissues and the effects on the biological functions of cervical cancer SiHa cells.
MATERIALS AND METHODS: Through transient transfection of SiHa cells with mature miR-101 sequences, the effects on apoptosis, proliferation, and cell cycle were evaluated by real-time PCR, CCK-8 assay, and flow cytometry.
RESULTS: Significantly less miR-101 was expressed in cervical cancer tissues than that in normal cervical tissues. miR-101 was overexpressed in SiHa cells through transient transfection of miR-101 mimics. CCK-8 assay and flow cytometry showed that miR-101 overexpression significantly inhibited cell proliferation, pro- moted apoptosis, and arrested them in the G(l)/S phase. Real-time PCR exhibited that Mcl-i and c-Fos mRNA expressions significantly decreased.
CONCLUSION: miR-101 significantly reduced the viability of SiHa cells as a potential anti-oncogene.
MATERIALS AND METHODS: Through transient transfection of SiHa cells with mature miR-101 sequences, the effects on apoptosis, proliferation, and cell cycle were evaluated by real-time PCR, CCK-8 assay, and flow cytometry.
RESULTS: Significantly less miR-101 was expressed in cervical cancer tissues than that in normal cervical tissues. miR-101 was overexpressed in SiHa cells through transient transfection of miR-101 mimics. CCK-8 assay and flow cytometry showed that miR-101 overexpression significantly inhibited cell proliferation, pro- moted apoptosis, and arrested them in the G(l)/S phase. Real-time PCR exhibited that Mcl-i and c-Fos mRNA expressions significantly decreased.
CONCLUSION: miR-101 significantly reduced the viability of SiHa cells as a potential anti-oncogene.