Медицинская библиотека · Japanese journal of clinical oncology · 2000

Clinical significance of major and minor bcr/abl chimeric transcripts in essential thrombocythemia.

Yamada H, Murakami T, Kaneda T, Tadachi M, Utsumi M, Minami S, Hamaguchi M, Kasai M, Kodera Y, Ohashi H, Morishita Y, Terasawa T, Yamasaki Y, Kamiya Y, Hattori M, Yamanaka K, Tsushita K, Shimoyama M

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Аннотация

BACKGROUND

Contradictory results have been reported in terms of detecting bcr/abl transcripts in patients with essential thrombocythemia. The aim of this study was to investigate whether the bcr/abl transcript could be found in patients with essential thrombocythemia (ET).

METHODS

The bcr/abl transcript was amplified by the RT-nested PCR method using RNA extract from leukocytes taken from 14 essential thrombocythemia patients. The amplified DNAs were electrophoresed in 1% agarose and visualized with ethidium bromide. The DNA bands associated with the bcr/abl transcript were then extracted and followed by DNA sequence analysis.

RESULTS

Major bcr/abl transcripts of the b3a2 type and minor ones of the e1a2 type were found in one and two ET patients, respectively. The incidence of bcr/abl transcripts was 21.4% (three of 14 patients).

CONCLUSION

Our experiments confirmed that bcr/abl transcripts are present in some patients with essential thrombocythemia.

Для цитирования: Yamada H, Murakami T, Kaneda T, Tadachi M, Utsumi M, Minami S, Hamaguchi M, Kasai M, Kodera Y, Ohashi H, Morishita Y, Terasawa T, Yamasaki Y, Kamiya Y, Hattori M, Yamanaka K, Tsushita K, Shimoyama M. Clinical significance of major and minor bcr/abl chimeric transcripts in essential thrombocythemia.. Japanese journal of clinical oncology. 2000. DOI: 10.1093/jjco/hyd122.

Предметные рубрики MeSH

Adult Aged Female Fusion Proteins, bcr-abl Humans Male Middle Aged Oncogene Proteins, Fusion Recombinant Fusion Proteins Reverse Transcriptase Polymerase Chain Reaction Thrombocythemia, Essential Transcription, Genetic

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