Oral diseases · 2008
Differential gene expression profiles of normal human parotid and submandibular glands.
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Sun Q-F, Sun Q-H, Du J, Wang S
Аннотация
BACKGROUND: Parotid and submandibular glands have different properties including characteristics of the secreted saliva and tumor incidences. The differences in properties of parotid and submandibular glands are not clear from a genetic viewpoint.
OBJECTIVE: To study differential gene expression profiles between normal human parotid and submandibular glands.
MATERIALS AND METHODS: Three pairs of normal parotid and submandibular glands were obtained. RNA was extracted from these samples. After reverse transcription, the cDNA was in vitro-transcribed to produce biotin-labeled cRNA. The purified biotin-labeled cRNA samples were hybridized to microarray chips.
RESULTS: Among the 54 675 tested transcripts, 47 transcripts were upregulated at least twofold in the parotid gland compared with the submandibular gland, including tumor-associated genes (pleiotrophin, WNT5A, ABCC1) and transport-associated genes (SLCO1A2, SLC13A5, KCNJ15). Ninety-eight transcripts were upregulated at least twofold in the submandibular gland compared with the parotid gland, including the chloride channel CFTR and mucin-associated genes that belong to the starch and sucrose metabolism pathway (GalNAc-T4, GalNAc-T7 and GalNAc-T13). Quantitative real-time reverse transcriptase-polymerase chain reaction (RT-PCR) analysis of nine differentially expressed genes confirmed the microarray results.
CONCLUSION: This study revealed the different gene expression profiles of normal human parotid and submandibular glands, providing a genetic basis for their differing properties.
OBJECTIVE: To study differential gene expression profiles between normal human parotid and submandibular glands.
MATERIALS AND METHODS: Three pairs of normal parotid and submandibular glands were obtained. RNA was extracted from these samples. After reverse transcription, the cDNA was in vitro-transcribed to produce biotin-labeled cRNA. The purified biotin-labeled cRNA samples were hybridized to microarray chips.
RESULTS: Among the 54 675 tested transcripts, 47 transcripts were upregulated at least twofold in the parotid gland compared with the submandibular gland, including tumor-associated genes (pleiotrophin, WNT5A, ABCC1) and transport-associated genes (SLCO1A2, SLC13A5, KCNJ15). Ninety-eight transcripts were upregulated at least twofold in the submandibular gland compared with the parotid gland, including the chloride channel CFTR and mucin-associated genes that belong to the starch and sucrose metabolism pathway (GalNAc-T4, GalNAc-T7 and GalNAc-T13). Quantitative real-time reverse transcriptase-polymerase chain reaction (RT-PCR) analysis of nine differentially expressed genes confirmed the microarray results.
CONCLUSION: This study revealed the different gene expression profiles of normal human parotid and submandibular glands, providing a genetic basis for their differing properties.
Предметные рубрики MeSH
Carrier ProteinsCystic Fibrosis Transmembrane Conductance RegulatorCytokinesDrug Resistance, MultipleGene Expression ProfilingGlucuronosyltransferaseHumansMucinsATP-Binding Cassette, Sub-Family C ProteinsN-AcetylgalactosaminyltransferasesOligonucleotide Array Sequence AnalysisOrganic Anion TransportersParotid GlandPotassium Channels, Inwardly RectifyingProto-Oncogene ProteinsRNAReverse Transcriptase Polymerase Chain ReactionReverse TranscriptionSubmandibular GlandSymportersUp-RegulationWnt ProteinsWnt-5a ProteinPolypeptide N-acetylgalactosaminyltransferase