[Determination of the dynamics of activity and stability of PSA total samples in semen samples on gauze, diluted with deionized water, using the method of quantitative enzyme immunoassay].

OBJECTIVE

Establishment of the dynamics of PSA total activity and stability in human semen samples dried on gauze and diluted with deionized water.

MATERIAL AND

METHODS

We studied samples of human seminal fluid, dried on gauze and diluted with deionized water. Measurements in the samples were carried out 5 times during the working day. The study was carried out using a quantitative enzyme-linked immunosorbent assay using the domestic «Alcorbio».

RESULTS

Quantitative indicators of a decrease in PSA total activity in the studied samples for 6 hours at a temperature of +18-20 °C and the suitability of the samples for research by enzyme-linked immunosorbent assay were established.

CONCLUSION

The data obtained can be used when conducting forensic biological examinations and studies to determine the presence of sperm on physical evidence using PSA total using quantitative enzyme-linked immunosorbent assay.