Journal of gastroenterology and hepatology · 2025
m6A-Modified BASP1 Regulates IL6 Expression to Induce TAM Infiltration to Promote Gastric Cancer Progression.
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Liu Guanghui, Liu Yuanhua, Zhao Hongchao, Sun Junfeng, Yang Hongwei, Ding Qingqing
Аннотация
OBJECTIVE: Gastric cancer (GC) is aggressive with poor prognosis. This study explores m6A's role in BASP1 regulation and its effects on GC via IL6, M2 macrophages, and PI3K/AKT.
METHODS: This study analyzed GC data from TCGA, used GC cell lines (Hs746T, KATO III, MKN-45, NCI-N87) and nude mice, modulated BASP1 via lentivirus, assessed cell functions with CCK8, wound healing, Transwell, and flow cytometry, investigated m6A's role with MeRIP-PCR and RNA pulldown, and evaluated macrophage polarization and IL6 with co-culture and ELISA. The involvement of the PI3K/AKT signaling pathway was examined using WB and specific inhibitors.
RESULTS: BASP1 is higher in GC tissues and is linked to poor prognosis. Knockdown inhibited cell viability, migration, and invasion and promoted apoptosis. m6A regulated BASP1 via mRNA stability (IGF2BP2 key). BASP1 regulated IL6 via PI3K/AKT, affecting M2 macrophages. Overexpression in vivo accelerated tumor growth and increased M2 macrophages and IL6.
CONCLUSIONS: BASP1's role in GC progression and tumor microenvironment modulation via IL6, M2 macrophages, and PI3K/AKT has been elucidated. This provides a basis for new GC therapies targeting BASP1.
METHODS: This study analyzed GC data from TCGA, used GC cell lines (Hs746T, KATO III, MKN-45, NCI-N87) and nude mice, modulated BASP1 via lentivirus, assessed cell functions with CCK8, wound healing, Transwell, and flow cytometry, investigated m6A's role with MeRIP-PCR and RNA pulldown, and evaluated macrophage polarization and IL6 with co-culture and ELISA. The involvement of the PI3K/AKT signaling pathway was examined using WB and specific inhibitors.
RESULTS: BASP1 is higher in GC tissues and is linked to poor prognosis. Knockdown inhibited cell viability, migration, and invasion and promoted apoptosis. m6A regulated BASP1 via mRNA stability (IGF2BP2 key). BASP1 regulated IL6 via PI3K/AKT, affecting M2 macrophages. Overexpression in vivo accelerated tumor growth and increased M2 macrophages and IL6.
CONCLUSIONS: BASP1's role in GC progression and tumor microenvironment modulation via IL6, M2 macrophages, and PI3K/AKT has been elucidated. This provides a basis for new GC therapies targeting BASP1.
Предметные рубрики MeSH
Stomach NeoplasmsHumansInterleukin-6Disease ProgressionAnimalsPhosphatidylinositol 3-KinasesCell Line, TumorMice, NudeSignal TransductionTumor MicroenvironmentProto-Oncogene Proteins c-aktMacrophagesCell MovementGene Expression Regulation, NeoplasticMembrane ProteinsApoptosisNerve Tissue ProteinsNeoplasm InvasivenessMiceRepressor Proteins