Journal of environmental pathology, toxicology and oncology : official organ of the International Society for Environmental Toxicology and Cancer · 2025
Increased hsa_circ_0075829 Facilitates the Progression of Gastric Cancer and Its Relationship with Helicobacter Pylori Infection.
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Li Zhentao, Zhang Haihan, Wang Zhuangmei
Аннотация
BACKGROUND: Circular RNAs (circRNAs) play a pivotal part in the advancement of multiple tumors. Nonetheless, the influence of Helicobacter pylori (H. pylori) infection on the expression of circRNA in gastric cancer remains less studied.
AIM: The objective of this research is to clarify the function and underlying mechanism of circ_0075829 in the context of gastric cancer and its relation to H. pylori infection.
METHODS: The circ_0075829 expression in tissue specimens and cells was evaluated utilizing quantitative real-time PCR (RT-qPCR). H. pylori infection and transfection were treated in gastric cancer cells to measure circ_0075829 expression changes and their effects on cellular behaviors. Kaplan-Meier curve was conducted to assess the clinical prognostic performance of circ_0085729 in patients with gastric cancer. Bioinformatics and dua-luciferase reporter assay were performed to validate the downstream miRNA and mRNA. CCK-8 proliferation assay and transwell experiments were carried out to investigate the influence of circ_0075829-miR-149-5p-AGO1 on cellular activities.
RESULTS: Circ_0075829 expression was increased in gastric cancer and correlated with several clinical characteristics and shorter overall survival. Besides, H. pylori infection increased circ_0075829 expression in cancer cells. miR-149-5p was a direct target miRNA of circ_0075829 and was downregulated in gastric cancer. Downregulation of circ_0075829 could repress the proliferative abilities, migratory capacities, and invasion potential of cancer cells by mediating miR-149-5p to regulate AGO1 expression.
CONCLUSION: Circ_0075829 seems to play an oncogenic role in H. pylori-associated gastric malignancies and may serve as a promising indicator for predicting outcomes in gastric cancer, which underpins a more theoretical basis for the progression of new therapeutic approaches to treating gastric cancer.
AIM: The objective of this research is to clarify the function and underlying mechanism of circ_0075829 in the context of gastric cancer and its relation to H. pylori infection.
METHODS: The circ_0075829 expression in tissue specimens and cells was evaluated utilizing quantitative real-time PCR (RT-qPCR). H. pylori infection and transfection were treated in gastric cancer cells to measure circ_0075829 expression changes and their effects on cellular behaviors. Kaplan-Meier curve was conducted to assess the clinical prognostic performance of circ_0085729 in patients with gastric cancer. Bioinformatics and dua-luciferase reporter assay were performed to validate the downstream miRNA and mRNA. CCK-8 proliferation assay and transwell experiments were carried out to investigate the influence of circ_0075829-miR-149-5p-AGO1 on cellular activities.
RESULTS: Circ_0075829 expression was increased in gastric cancer and correlated with several clinical characteristics and shorter overall survival. Besides, H. pylori infection increased circ_0075829 expression in cancer cells. miR-149-5p was a direct target miRNA of circ_0075829 and was downregulated in gastric cancer. Downregulation of circ_0075829 could repress the proliferative abilities, migratory capacities, and invasion potential of cancer cells by mediating miR-149-5p to regulate AGO1 expression.
CONCLUSION: Circ_0075829 seems to play an oncogenic role in H. pylori-associated gastric malignancies and may serve as a promising indicator for predicting outcomes in gastric cancer, which underpins a more theoretical basis for the progression of new therapeutic approaches to treating gastric cancer.