Pest management science · 2025
MicroRNA Lse-miR-2944c-3p regulates female reproduction in Lasioderma serricorne by targeting the heat shock protein LsHSP70-3.
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Xu Kang-Kang, Liang Chao-Shan, Yan Yi, Liu De-Qian, Yang Hong, Wan Fang-Hao, Yang Wen-Jia
Аннотация
BACKGROUND: The reproductive plasticity of female insects is governed by the complex structure and function of their ovaries. However, the molecular mechanisms underlying ovarian development in Lasioderma serricorne, a globally significant pest of stored products, remain largely unexplored. The role of microRNAs (miRNAs) in regulating genes associated with reproduction remains poorly characterized.
RESULTS: This study investigated the regulatory mechanisms involving the LsHSP70-3 gene, which encodes a heat shock protein, and its associated miRNA in the reproductive processes of female L. serricorne. RNA interference-mediated knockdown of LsHSP70-3 markedly impaired ovarian development and reduced female fecundity. In silico predictions, validated by quantitative real-time polymerase chain reaction, identified Lse-miR-2944c-3p as the cognate miRNA targeting LsHSP70-3. Dual-luciferase reporter assays and RNA pull-down experiments confirmed the interaction between Lse-miR-2944c-3p and the 3' untranslated region of LsHSP70-3, resulting in the repression of its expression. Microinjection of Lse-miR-2944c-3p mimics led to reduced expression of LsHSP70-3 and down-regulation of vitellogenin (Vg) and Vg receptor (VgR) transcripts, culminating in impaired ovarian development, decreased oviposition, and reduced egg hatchability. Conversely, administration of an Lse-miR-2944c-3p inhibitor elevated the expression of LsHSP70-3, LsVg, and LsVgR, thereby promoting oogenesis and increasing egg production. Rescue experiments involving the co-injection of the miRNA inhibitor and dsLsHSP70-3 corroborated that Lse-miR-2944c-3p modulates reproduction by fine-tuning LsHSP70-3 expression.
CONCLUSION: The findings suggested that LsHSP70-3 deficiency impairs reproductive development in L. serricorne females. Lse-miR-2944c-3p and its target, LsHSP70-3, represent potential molecular targets for the control of L. serricorne. © 2025 Society of Chemical Industry.
RESULTS: This study investigated the regulatory mechanisms involving the LsHSP70-3 gene, which encodes a heat shock protein, and its associated miRNA in the reproductive processes of female L. serricorne. RNA interference-mediated knockdown of LsHSP70-3 markedly impaired ovarian development and reduced female fecundity. In silico predictions, validated by quantitative real-time polymerase chain reaction, identified Lse-miR-2944c-3p as the cognate miRNA targeting LsHSP70-3. Dual-luciferase reporter assays and RNA pull-down experiments confirmed the interaction between Lse-miR-2944c-3p and the 3' untranslated region of LsHSP70-3, resulting in the repression of its expression. Microinjection of Lse-miR-2944c-3p mimics led to reduced expression of LsHSP70-3 and down-regulation of vitellogenin (Vg) and Vg receptor (VgR) transcripts, culminating in impaired ovarian development, decreased oviposition, and reduced egg hatchability. Conversely, administration of an Lse-miR-2944c-3p inhibitor elevated the expression of LsHSP70-3, LsVg, and LsVgR, thereby promoting oogenesis and increasing egg production. Rescue experiments involving the co-injection of the miRNA inhibitor and dsLsHSP70-3 corroborated that Lse-miR-2944c-3p modulates reproduction by fine-tuning LsHSP70-3 expression.
CONCLUSION: The findings suggested that LsHSP70-3 deficiency impairs reproductive development in L. serricorne females. Lse-miR-2944c-3p and its target, LsHSP70-3, represent potential molecular targets for the control of L. serricorne. © 2025 Society of Chemical Industry.