Klinische Monatsblatter fur Augenheilkunde · 2001
Cyclic AMP and anionic currents in porcine ciliary epithelium.
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Fleischhauer J C, Bény J L, Flammer J, Haefliger I O
Аннотация
INTRODUCTION: To investigate whether in the ciliary epithelium of isolated porcine ciliary body cyclic 3',5' adenosine monophosphate (cAMP) activates transmembrane anionic currents.
METHODS: Changes in membrane potential induced either by the adenylcyclase activator forskolin (10 microM; n = 4) or the stable membrane permeable cAMP analog 8-bromo-adenosine 3',5'-cyclic monophosphothioate (8-br-cAMP; 30 microM; n = 4) were measured with intracellular microelectrodes. The effect of the drugs were assessed in the absence or in the presence of the non-selective anionic channel/transporter inhibitor diisothiocyanatostilbene-2,2' disulfonic acid (DIDS; 1 mM; n = 4).
RESULTS: Significant (p < 0.001) membrane potential depolarization were induced by both forskolin (11.8 +/- 0.3 mV) or 8-br-cAMP (9.3 +/- 0.4 mV). In the presence of DIDS, a significant (p < 0.001) inhibition of the depolarization evoked by forskolin (0.9 +/- 1.1 mV) and 8-bromo-cAMP (0.7 +/- 0.2 mV) was observed.
CONCLUSIONS: In the ciliary epithelium of isolated porcine ciliary body cAMP induces membrane potential depolarization through a process that could involve anionic channels.
METHODS: Changes in membrane potential induced either by the adenylcyclase activator forskolin (10 microM; n = 4) or the stable membrane permeable cAMP analog 8-bromo-adenosine 3',5'-cyclic monophosphothioate (8-br-cAMP; 30 microM; n = 4) were measured with intracellular microelectrodes. The effect of the drugs were assessed in the absence or in the presence of the non-selective anionic channel/transporter inhibitor diisothiocyanatostilbene-2,2' disulfonic acid (DIDS; 1 mM; n = 4).
RESULTS: Significant (p < 0.001) membrane potential depolarization were induced by both forskolin (11.8 +/- 0.3 mV) or 8-br-cAMP (9.3 +/- 0.4 mV). In the presence of DIDS, a significant (p < 0.001) inhibition of the depolarization evoked by forskolin (0.9 +/- 1.1 mV) and 8-bromo-cAMP (0.7 +/- 0.2 mV) was observed.
CONCLUSIONS: In the ciliary epithelium of isolated porcine ciliary body cAMP induces membrane potential depolarization through a process that could involve anionic channels.