IGF-I and INS receptor expression in the salivary glands of diabetic Nod mice submitted to long-term insulin treatment.

This work aimed to characterize the IGF-I and INS receptor expression in the salivary glands of Nod mice, correlating to therapeutic effects of insulin treatment on these receptors. Nod mice were divided into: Groups 1 and 2 (diabetic), Groups 3 and 4 (diabetic with insulin treatment) and Group 5 (non-diabetic). Fragments from the salivary glands were processed for immunohistochemical analysis. The results showed that the prolonged diabetic state led to a steadily increased IGF-I receptor expression. INS receptor expression was gradually decreased. It was concluded that not only was the IGF-I receptor expression affected by the diabetic state but also the INS receptor expression. The period of the diabetic state was directly related to changes in the expression of these receptors. In spite of the insulin treatment having recovered the glycaemic levels, the expression of INS and the IGF-I receptors did not reach the standard level, which certainly hampered glandular function.