Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery · 2007
Comprehensive loss of heterozygosity analysis and identification of a novel hotspot at 3p21 in salivary gland neoplasms.
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Honjo Noriyasu, Gunduz Mehmet, Fukushima Kunihiro, Cengiz Beyhan, Beder Levent B, Gunduz Esra, Nagatsuka Hitoshi, Xiao Jing, Shimizu Kenji, Nishizaki Kazunori
Аннотация
OBJECTIVES: We sought to assess loss of heterozygosity (LOH) profiles of 3p, 6q, 8q, 10q, 12q, 13q, and 17p and to identify the tumor suppressor genes involved in salivary gland neoplasms.
STUDY DESIGN: LOH analysis was performed using 26 microsatellite markers by polymerase chain reaction-polyacrylamide gel electrophoresis method in 20 benign and 6 malignant salivary gland tumors.
RESULTS: Overall, LOH was detected in at least one informative locus in 18 of 20 (90%) of benign tumors and in all of 6 cases of malignant tumors. High LOH frequencies were revealed at the loci D3S1307 (22%, 3p26), D3S966 (41%, 3p21), D6S255 (27%, 6q25), D8S166 (25%, 8q12), D8S199 (21%, 8q24), and D10S1765 (28%, 10q23) in benign tumors, defining the hotspot regions for putative tumor suppressor genes.
CONCLUSIONS AND SIGNIFICANCE: The hotspot regions defined by the present study suggest that new tumor suppressor genes related to the development of salivary gland tumors may reside at several chromosomal loci, including loci at 3p, 6q, 8q and 10q.
STUDY DESIGN: LOH analysis was performed using 26 microsatellite markers by polymerase chain reaction-polyacrylamide gel electrophoresis method in 20 benign and 6 malignant salivary gland tumors.
RESULTS: Overall, LOH was detected in at least one informative locus in 18 of 20 (90%) of benign tumors and in all of 6 cases of malignant tumors. High LOH frequencies were revealed at the loci D3S1307 (22%, 3p26), D3S966 (41%, 3p21), D6S255 (27%, 6q25), D8S166 (25%, 8q12), D8S199 (21%, 8q24), and D10S1765 (28%, 10q23) in benign tumors, defining the hotspot regions for putative tumor suppressor genes.
CONCLUSIONS AND SIGNIFICANCE: The hotspot regions defined by the present study suggest that new tumor suppressor genes related to the development of salivary gland tumors may reside at several chromosomal loci, including loci at 3p, 6q, 8q and 10q.
Предметные рубрики MeSH
AdenolymphomaAdenoma, PleomorphicAdultAgedCarcinomaCarcinoma, Squamous CellChromosome MappingChromosomes, Human, Pair 10Chromosomes, Human, Pair 12Chromosomes, Human, Pair 13Chromosomes, Human, Pair 17Chromosomes, Human, Pair 3Chromosomes, Human, Pair 6Chromosomes, Human, Pair 8FemaleGenes, Tumor SuppressorHumansLoss of HeterozygosityMaleMicrosatellite RepeatsMiddle AgedParotid NeoplasmsSubmandibular Gland Neoplasms