[In vitro study of the lipase activity of bacterial strains isolated from the rabbit cecum on C14-labeled triolein].

The lipase activity of bacterial species isolated from the caecum of conventional rabbits was studied by means of two groups of tests: (1) bacteriologic isolation and an identification of nine aerobic and anaerobic clones and (2) incubation of each of these clones in the appropriate medium in the presence of labelled triolein (oleyl 1-14C), a biochemical analysis of the radioactivity of the fractions obtained. The main results can be expressed in the following way: maximum hydrolysis of glycerides is achieved by two species: Bacillus licheniformis and Clostridium sporogenes. The reaction is proportional to the incubation time for incubations of less than 8 h. The release of fatty acids is concomitant to the synthesis of phospholipids and of fatty acid methyl esters.