Phytomedicine : international journal of phytotherapy and phytopharmacology · 2025
Serum pharmacochemistry and network pharmacology reveal the effect and mechanism of licorice extracts on colorectal cancer.
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Li Yamei, Wen Junya, Deng Kaili, Li Yuanyuan, Wu Yufan, Wu Yuge, Wang Zhuxian, Zhai Dan, Xu Yuxuan, Zhu Hongxia, Liu Qiang
Аннотация
BACKGROUND: Colorectal cancer (CRC) is a prevalent malignant tumor. As per the principles of Traditional Chinese Medicine (TCM), the primary pathogenesis of CRC is associated with a deficiency in vital energy. Licorice, known for its spleen-tonifying and qi-enhancing efficacy, shows potential for preventing and treating CRC. Nevertheless, the pharmacological foundation and underlying mechanisms of licorice in CRC treatment are still not fully understood.
METHODS: UPLC-Q-Exactive Orbitrap MS was employed to analyze the components of Licorice extracts (LEs) and the substances absorbed into the bloodstream. The key active substances and potential mechanisms were screened using network pharmacology and docking techniques. The selected results were validated in vivo and in vitro.
RESULTS: 43 compounds of LEs were absorbed. LEs demonstrated inhibitory effects on the development and progression of CRC in mice and cells. The study revealed that the absorbed components of LEs triggered tumor cell apoptosis, decreased the expression of EGFR and HIF-1α, and suppressed the activation of the RAS/RAF/MEK/ERK signaling pathway.
CONCLUSION: LEs exert anti-CRC effects through apoptosis induction and RAS/RAF/MEK/ERK pathway inhibition. These findings demonstrate the great potential of TCM in the treatment of CRC and support its broader clinical application in combination chemotherapy and targeted therapy.
METHODS: UPLC-Q-Exactive Orbitrap MS was employed to analyze the components of Licorice extracts (LEs) and the substances absorbed into the bloodstream. The key active substances and potential mechanisms were screened using network pharmacology and docking techniques. The selected results were validated in vivo and in vitro.
RESULTS: 43 compounds of LEs were absorbed. LEs demonstrated inhibitory effects on the development and progression of CRC in mice and cells. The study revealed that the absorbed components of LEs triggered tumor cell apoptosis, decreased the expression of EGFR and HIF-1α, and suppressed the activation of the RAS/RAF/MEK/ERK signaling pathway.
CONCLUSION: LEs exert anti-CRC effects through apoptosis induction and RAS/RAF/MEK/ERK pathway inhibition. These findings demonstrate the great potential of TCM in the treatment of CRC and support its broader clinical application in combination chemotherapy and targeted therapy.