Histomorphometric analysis of the effects of creatine on rat myometrium.

OBJECTIVE

To analyze the myometrial thickness of rats subjected to creatine (Cr) ingestion.

STUDY

DESIGN

A total of 14 rats was equally divided into the control group (ConGr) receiving 1 ml potable water and the creatine group (CrGr) subjected to the ingestion of 1.6 g/kg Cr diluted in 1 ml potable water. At the end of 8 weeks, the animals were anesthetized (xylazine and ketamine) and sacrificed, the uteri and ovaries stained with hematoxylin and eosin, the thickness of both the myometrium and the epithelium measured and the follicles counted.

RESULTS

Analysis revealed a significant increase in thickness of the myometrium in the CrGr (272.26 ± 66.71μm) contrasted with that from the ConGr (160.76 ± 35.65μm), CrGr > ConGr (p < 0001).

CONCLUSION

Our data suggest that Cr changed myometrial morphology in rats by enhancing myometrial thickness, but its action mechanism in the smooth muscle is still unclear.