[Incorporation of 3H-estradiol into elements of the tanycytic and ciliated ependyma of the 3d cerebral ventricle in experiments in vitro].

The label incorporation into the arcuate nucleus neurons, the tanicytic and ciliary ependyma of the mediobasal hypotahalamus, and also into the underlying nerve tissue after in vitro incubation of male Wistar rats hypothalamic tissue in the presence of 3H-estradiol was studied autoradiographically. After incubation at 37 degrees C, along with the nuclear localization of the label in the neutrons of the arcuate nucleus, characteristic of estradiol, high radioactivity was revealed in tanicytes; the label incorporation into beta-tanicytes was always higher than into the alpha2-tanicytes. Subsequent incubation of the tissue with nonlabeled estradiol led to reduction of beta-tanicytes radioactivity; as to ciliary ependyma and alpha2-tanicytes radioactivity--it rose. Reduction of incubation temperature to 22 degrees C failed to alter the character of the label incorporation into tanicytes. The data obtained confirmed the possibility of participation of the mediobasal hypothalamus tanicytes in the realization of the estradiol action of the hypothalamus.