Functionalization of Fe3O4/rGO magnetic nanoparticles with resveratrol and in vitro DNA interaction.

Based on the previous research, we found that the magnetic nanocomposite Fe3O4/rGO (reduced graphene oxide) has a good drug loading effect. Therefore, in this paper, we studied the positive role of Fe3O4/rGO as a drug carrier in the interaction between resveratrol (RES) and calf-thymus DNA (ct-DNA). The fluorescence experiment is used to evaluate by the Stern-Volmer equation, the quenching constant of RES - ct-DNA system with and without Fe3O4/rGO decreases with the increasing temperature. It was found the quenching mode of RES - ct-DNA and Fe3O4/rGO - RES - ct-DNA systems were all static quenching, but the binding constant of RES -ct-DNA increased from 4.14 ± 0.21 × 104 L mol-1 to 10.12 ± 0.02 × 104 L mol-1. It was found that Fe3O4/rGO formed a ternary complex with RES and ct-DNA by ultraviolet spectrum (UV-vis), resonance light scattering experiments (RLS) and scanning electron microscope (SEM). Meanwhile, Fourier transform infrared (FT-IR) and circular dichroism (CD) experiments show that Fe3O4/rGO and Fe3O4/rGO loaded with RES have effect on the secondary structure of ct-DNA and change the conformation of ct-DNA. On the cellular level, the comet assay shows that Fe3O4/rGO and Fe3O4/rGO - RES could not cause DNA strand break to the mouse hepatocytes after 24 co-incubation. These results confirm that Fe3O4/rGO nanocomposites have good application potential, which can be used as a good drug carrier in a wide range of therapeutic methods.