Bisphenol A promotes the proliferation of leiomyoma cells by GPR30-EGFR signaling pathway.

AIM: To study the molecular mechanism of G protein-coupled receptor 30-epidermal growth factor receptor (GPR30-EGFR) signaling pathway on the proliferation of leiomyoma cells exposed with bisphenol A.

METHODS

Primary cultures and subcultures of human uterine leiomyoma (UL) cells. The expressions of messenger RNA and proteins of GPR30 and EGFR in 15 leiomyoma tissue specimens and all groups were detected by real-time quantitative polymerase chain reaction assay and Western blot assay. The protein of mitogen-activated protein kinases (MAPK)/extracellular signal-regulated kinases (ERK)/c-fos signaling pathway members was detected by Western blot assay.

RESULTS

Bisphenol A promoted the growth of UL cells and the expressions of GPR30, EGFR, c-fos and p-ERK1/2.

CONCLUSION

Bisphenol A was found to be a promoter specifically to proliferate the human UL cells by activating the transcription and translation of GPR30-EGFR and MAPK/ERK/c-fos signaling pathway members.