Letters in applied microbiology · 2001
Comparative study of the influence of melatonin and vitamin E on the surface characteristics of Escherichia coli.
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Uberos J, Augustin C, Liébana J, Molina A, Muñoz-Hoyos A
Аннотация
AIMS: Melatonin is a hormone produced by the pineal gland and that affects the response of various cell membranes to an oxidative stimulus.
METHODS AND RESULTS: The present study evaluates the hydrophobic characteristics of Escherichia coli in response to melatonin (100 nmol l(-1), 200 micromol l(-1)) and to vitamin E (5 mg dl(-1)). A reduction was found in the surface hydrophobicity of E. coli at concentrations of 200 micromol l(-1) melatonin in a Müeller-Hinton (MH) broth. These effects were modified when a protein synthesis inhibitor (chloramphenicol) was added at sub-lethal concentrations to the broth. Vitamin E produced a greater diminution in surface hydrophobicity than melatonin. The adherence of E. coli to nitrocellulose filters increased in the presence of melatonin + chloramphenicol, and vitamin E. The effects observed were independent of the concentration of iron in the broth.
CONCLUSION: Oxidative stress plays an important role in modifying the surface characteristics of E. coli, which could affect the micro-organism's capacity to adhere to epithelia.
SIGNIFICANCE AND IMPACT OF THE STUDY: We think that the oxide reduction potential of the host may be a determinant factor in the bacterial colonization of animal tissue.
METHODS AND RESULTS: The present study evaluates the hydrophobic characteristics of Escherichia coli in response to melatonin (100 nmol l(-1), 200 micromol l(-1)) and to vitamin E (5 mg dl(-1)). A reduction was found in the surface hydrophobicity of E. coli at concentrations of 200 micromol l(-1) melatonin in a Müeller-Hinton (MH) broth. These effects were modified when a protein synthesis inhibitor (chloramphenicol) was added at sub-lethal concentrations to the broth. Vitamin E produced a greater diminution in surface hydrophobicity than melatonin. The adherence of E. coli to nitrocellulose filters increased in the presence of melatonin + chloramphenicol, and vitamin E. The effects observed were independent of the concentration of iron in the broth.
CONCLUSION: Oxidative stress plays an important role in modifying the surface characteristics of E. coli, which could affect the micro-organism's capacity to adhere to epithelia.
SIGNIFICANCE AND IMPACT OF THE STUDY: We think that the oxide reduction potential of the host may be a determinant factor in the bacterial colonization of animal tissue.