Fertility and sterility · 2011
Expression of dysadherin in the human male reproductive tract and in spermatozoa.
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Gabrielli Nieves María, Veiga María Florencia, Matos María Laura, Quintana Silvina, Chemes Héctor, Blanco Gustavo, Vazquez-Levin Mónica Hebe
Аннотация
OBJECTIVE: To study expression of dysadherin in human testis, epididymis, and spermatozoa.
DESIGN: Prospective study.
SETTING: Basic research laboratory.
PATIENT(S): Testis, epididymis, and testicular spermatozoa from patients under treatment and semen from volunteer donors.
INTERVENTION(S): Reverse transcription-polymerase chain reaction, immunohistochemistry, immunocytochemistry, and Western immunoblotting.
MAIN OUTCOME MEASURE(S): Dysadherin messenger RNA (mRNA) analysis in testis, epididymis, and ejaculated spermatozoa, immunohistochemistry of both tissues, Western immunoblotting of tissue/cell extracts, and immunocytochemistry of spermatozoa.
RESULT(S): Dysadherin mRNA was found in testis, epididymis, and ejaculated spermatozoa. Whereas testis and spermatozoa exhibited a distinctive 91-kDa protein form, the epididymis showed a 50-kDa moiety, also found in MDA-MB-231 breast cancer cells. Nucleotide sequence analysis revealed >99% homology between testicular and somatic cell mRNA, suggesting differential protein glycosylation. Dysadherin was immunodetected in round spermatids and testicular/ejaculated spermatozoa. It localizes to the acrosomal region and flagellum and colocalized with E-cadherin in the head and with the Na(+),K(+)-ATPase α4 subunit in the flagellum.
CONCLUSION(S): This is the first report on expression of dysadherin in the male gonad and in spermatozoa. Its colocalization with E-cadherin and Na(+),K(+)-ATPase leads us to postulate a role for dysadherin as a modulator of sperm function.
DESIGN: Prospective study.
SETTING: Basic research laboratory.
PATIENT(S): Testis, epididymis, and testicular spermatozoa from patients under treatment and semen from volunteer donors.
INTERVENTION(S): Reverse transcription-polymerase chain reaction, immunohistochemistry, immunocytochemistry, and Western immunoblotting.
MAIN OUTCOME MEASURE(S): Dysadherin messenger RNA (mRNA) analysis in testis, epididymis, and ejaculated spermatozoa, immunohistochemistry of both tissues, Western immunoblotting of tissue/cell extracts, and immunocytochemistry of spermatozoa.
RESULT(S): Dysadherin mRNA was found in testis, epididymis, and ejaculated spermatozoa. Whereas testis and spermatozoa exhibited a distinctive 91-kDa protein form, the epididymis showed a 50-kDa moiety, also found in MDA-MB-231 breast cancer cells. Nucleotide sequence analysis revealed >99% homology between testicular and somatic cell mRNA, suggesting differential protein glycosylation. Dysadherin was immunodetected in round spermatids and testicular/ejaculated spermatozoa. It localizes to the acrosomal region and flagellum and colocalized with E-cadherin in the head and with the Na(+),K(+)-ATPase α4 subunit in the flagellum.
CONCLUSION(S): This is the first report on expression of dysadherin in the male gonad and in spermatozoa. Its colocalization with E-cadherin and Na(+),K(+)-ATPase leads us to postulate a role for dysadherin as a modulator of sperm function.
Предметные рубрики MeSH
Acrosome ReactionBiopsyBreast NeoplasmsCadherinsCell Line, TumorEndothelial CellsEpididymisExocytosisGene ExpressionHumansIon ChannelsMaleMembrane GlycoproteinsMicrofilament ProteinsNeoplasm ProteinsProstatic NeoplasmsRNA, MessengerSodium-Potassium-Exchanging ATPaseSperm CapacitationSpermatozoaTestisUmbilical Veins