Медицинская библиотека · Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology · 2005

[Preliminary study on anti-periodontitis immunization with DNA vaccine].

Zhang Feng-qiu, Yang Lian-jia, Wu Zhi-fen

Аннотация на английскомСтатьяПеревод пока не подготовлен

Аннотация

OBJECTIVE

To observe the protection against periodontal bone loss in the Sprague-Dawley (SD) rats periodontitis model, with the recombined plasmid pcDNA3.1+/kgpcd as DNA gene vaccine.

METHODS

PcDNA3.1+/kgpcd was delivered into rats by submandibular gland-targeted injection. The anti-KGPcd sIgA in saliva was measured by indirect ELISA method. Immunohistochemistry staining was used to assess the protection in the animal model.

RESULTS

The level of specific anti-KGPcd sIgA in saliva of the experimental group was significantly higher than that of control group. HE staining showed that immunization with recombined plasmid pcDNA3.1+/kgpcd could protect or minimize tissue destruction caused by subsequent P. gingivalis challenge in the rat model.

CONCLUSIONS

The results indicate that pcDNA3.1+/kgpcd was a good candidate for anti-periodontitis gene vaccine and could provide protection against Porphyromonas gingivalis-caused periodontitis in rat lesion model.

Для цитирования: Zhang Feng-qiu, Yang Lian-jia, Wu Zhi-fen. [Preliminary study on anti-periodontitis immunization with DNA vaccine].. Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology. 2005.

Предметные рубрики MeSH

Animals Bacterial Vaccines Immunoglobulin A, Secretory Periodontitis Porphyromonas gingivalis Rats Rats, Sprague-Dawley Vaccines, DNA

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