Implant dentistry · 2015
Conditioned Medium From Mesenchymal Stem Cells Enhances Early Bone Regeneration After Maxillary Sinus Floor Elevation in Rabbits.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Katagiri Wataru, Osugi Masashi, Kinoshita Kazuhiko, Hibi Hideharu
Аннотация
PURPOSE: Conditioned medium from stem cells contains growth factors and has a promising prospect for use in regenerative medicine. In this study, the effects of marrow-derived stem cell-conditioned medium (MSC-CM) on bone regeneration after maxillary sinus floor elevation were examined in rabbits.
MATERIALS AND METHODS: Rabbit bone marrow-derived stem cells (rMSCs) were cultured with MSC-CM for 48 hours, and their mobilization and proliferation were evaluated. Beta-tricalcium phosphate scaffolds were impregnated with MSC-CM and grafted in the rabbit maxillary sinus cavities. At 2, 4, and 8 weeks after transplantation, sections of the newly formed bone were evaluated histologically and by immunohistochemical staining.
RESULTS: MSC-CM increased the migration and proliferation of rMSCs and the early bone regeneration in rabbit sinus. Cell proliferation and vascularization were increased at 2 weeks after implantation of grafts impregnated with MSC-CM compared to controls, indicating that MSC-CM is effective at the early phase of bone regeneration.
CONCLUSION: MSC-CM is a promising novel therapeutic agent to promote bone regeneration after maxillary sinus floor elevation.
MATERIALS AND METHODS: Rabbit bone marrow-derived stem cells (rMSCs) were cultured with MSC-CM for 48 hours, and their mobilization and proliferation were evaluated. Beta-tricalcium phosphate scaffolds were impregnated with MSC-CM and grafted in the rabbit maxillary sinus cavities. At 2, 4, and 8 weeks after transplantation, sections of the newly formed bone were evaluated histologically and by immunohistochemical staining.
RESULTS: MSC-CM increased the migration and proliferation of rMSCs and the early bone regeneration in rabbit sinus. Cell proliferation and vascularization were increased at 2 weeks after implantation of grafts impregnated with MSC-CM compared to controls, indicating that MSC-CM is effective at the early phase of bone regeneration.
CONCLUSION: MSC-CM is a promising novel therapeutic agent to promote bone regeneration after maxillary sinus floor elevation.