Effect of Lacticaseibacillus Rhamnosu AFY02 on AOM/DSS-Induced Inflammation-Associated Colon Cancer in Mice and the Mechanism.

The aim of this study was to explore the effects of Lacticaseibacillus rhamnosu AFY02 (LR-AFY02), which was obtained from traditional fermented yogurt, on mice with inflammation-related AOM/DSS-induced colon cancer and the associated processes. The pathological results of the colon tissue of mice, the indicators of serum and tissue, and their expressions in the tissue were detected. The results showed that the use of LR-AFY02 significantly improved intestinal histopathological injury, decreased the incidence of intestinal tumors, downregulated the expression of IκBβ, p65, p50, p52, Bcl-2, and Bcl-xL in colon tissue, and increased the expression of Bid and caspase-8 in colon tissue. Compared with the model group, high concentration LR-AFY02H could reduce IL-1β, IL-6, TNF-α, NF-κB, and iNOS cytokine levels in serum and colon tissue. The ability to downregulate these cytokines was close to that of aspirin and stronger than that of low concentration LR-AFY02L. By controlling the NF-κB and apoptosis pathways, LR-AFY02 can help reduce intestinal inflammation and prevent the growth of intestinal tumors. These findings suggested that LR-AFY02 may have regulatory effects on colon cancer in mice and merits additional study.