Highly Sensitive and Efficient Screening of Streptococcus Agalactiae Through Improved Real-Time PCR Protocols and Optimized Analytical Parameters.

BACKGROUND

The detection of Streptococcus agalactiae colonization during pregnancy is important in order to prevent neonatal infections and reduce mortality rates.

METHODS

A highly sensitive (up to 1,000 UFC/mL), fully automated, and cost effective cylB specific real-time PCR protocol was developed for the detection of S. agalactiae in anal and vaginal swabs of pregnant women.

RESULTS

From the 62 test subjects, 24 were considered positive by PCR (38%) and 14 by culture (24%; p = 0.0015).

CONCLUSIONS

The observed discrepancies and the reduced turnaround time justifies molecular screening for S. agalactiae.