Cell stem cell · 2020
Derivation of Airway Basal Stem Cells from Human Pluripotent Stem Cells.
СтатьяПоддержка NIH: внешняя программаПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Hawkins Finn J, Suzuki Shingo, Beermann Mary Lou, Barillà Cristina, Wang Ruobing, Villacorta-Martin Carlos, Berical Andrew, Jean J C, Le Suer Jake, Matte Taylor, Simone-Roach Chantelle, Tang Yang, Schlaeger Thorsten M, Crane Ana M, Matthias Nadine, Huang Sarah X L, Randell Scott H, Wu Joshua, Spence Jason R, Carraro Gianni, Stripp Barry R, Rab Andras, Sorsher Eric J, Horani Amjad, Brody Steven L, Davis Brian R, Kotton Darrell N
Аннотация
The derivation of tissue-specific stem cells from human induced pluripotent stem cells (iPSCs) would have broad reaching implications for regenerative medicine. Here, we report the directed differentiation of human iPSCs into airway basal cells ("iBCs"), a population resembling the stem cell of the airway epithelium. Using a dual fluorescent reporter system (NKX2-1GFP;TP63tdTomato), we track and purify these cells as they first emerge as developmentally immature NKX2-1GFP+ lung progenitors and subsequently augment a TP63 program during proximal airway epithelial patterning. In response to primary basal cell medium, NKX2-1GFP+/TP63tdTomato+ cells display the molecular and functional phenotype of airway basal cells, including the capacity to self-renew or undergo multi-lineage differentiation in vitro and in tracheal xenografts in vivo. iBCs and their differentiated progeny model perturbations that characterize acquired and genetic airway diseases, including the mucus metaplasia of asthma, chloride channel dysfunction of cystic fibrosis, and ciliary defects of primary ciliary dyskinesia.