Aesthetic plastic surgery · 2026
Potential of Hair Follicle Stem Cell-Derived Exosomes Carrying miR-181a-5p to Promote the Telogen-to-Anagen Stage.
СтатьяНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Wang Yiping, Wu Wentao, Chen Jinwei, Xu Xiangping, Li Meiqi, Liao Xiangmin, Jia Chiyu, Chen Nian
Аннотация
BACKGROUND: Hair loss is a common clinical and aesthetic concern, and existing treatments remain limited by side effects, invasiveness, or unsatisfactory long-term efficacy. Stem cell-derived exosomes have emerged as a promising cell-free strategy for tissue regeneration. However, the hair-regenerative potential of hair follicle stem cell-derived exosomes (HFSC-Exos), particularly when enriched with miR-181a-5p, remains insufficiently explored.
METHODS: Commercially available rat hair follicle stem cells (HFSCs) were cultured, and exosomes were isolated from cells at passages 3-4 after transfection with miR-181a-5p mimics or negative control mimics. Exosomes were characterized by transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA), and western blotting. Twenty 7-week-old C57BL/6 mice, which were generally in telogen at this age, were randomly assigned to four groups (n = 5/group): PBS, Exos, ExosmiR-NC, and ExosmiR-181a-5p. After dorsal hair shaving, mice received local subcutaneous injections every other day for a total of five injections. Hair regrowth was evaluated by gross observation, histology, immunohistochemistry, and immunofluorescence.
RESULTS: HFSC-Exos accelerated the transition from telogen to anagen and promoted visible hair regrowth compared with PBS treatment. Among all groups, ExosmiR-181a-5p showed the strongest effect, with newborn hair coverage reaching approximately 90% on day 15, compared with approximately 40% in the Exos and ExosmiR-NC groups. In addition, Ki-67, Bcl-2, and β-catenin expression levels were significantly increased, whereas Bax expression was decreased in exosome-treated skin tissues (all P < 0.05), with the most pronounced changes observed in the ExosmiR-181a-5p group.
CONCLUSION: HFSC-Exos promoted the telogen-to-anagen transition and hair regrowth in vivo. Enrichment of miR-181a-5p enhanced this effect and was associated with activation of the Wnt/β-catenin pathway, although direct target validation remains to be established.
NO LEVEL ASSIGNED: This journal requires that authors assign a level of evidence to each submission to which Evidence-Based Medicine rankings are applicable. This excludes Review Articles, Book Reviews, and manuscripts that concern Basic Science, Animal Studies, Cadaver Studies, and Experimental Studies. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .
METHODS: Commercially available rat hair follicle stem cells (HFSCs) were cultured, and exosomes were isolated from cells at passages 3-4 after transfection with miR-181a-5p mimics or negative control mimics. Exosomes were characterized by transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA), and western blotting. Twenty 7-week-old C57BL/6 mice, which were generally in telogen at this age, were randomly assigned to four groups (n = 5/group): PBS, Exos, ExosmiR-NC, and ExosmiR-181a-5p. After dorsal hair shaving, mice received local subcutaneous injections every other day for a total of five injections. Hair regrowth was evaluated by gross observation, histology, immunohistochemistry, and immunofluorescence.
RESULTS: HFSC-Exos accelerated the transition from telogen to anagen and promoted visible hair regrowth compared with PBS treatment. Among all groups, ExosmiR-181a-5p showed the strongest effect, with newborn hair coverage reaching approximately 90% on day 15, compared with approximately 40% in the Exos and ExosmiR-NC groups. In addition, Ki-67, Bcl-2, and β-catenin expression levels were significantly increased, whereas Bax expression was decreased in exosome-treated skin tissues (all P < 0.05), with the most pronounced changes observed in the ExosmiR-181a-5p group.
CONCLUSION: HFSC-Exos promoted the telogen-to-anagen transition and hair regrowth in vivo. Enrichment of miR-181a-5p enhanced this effect and was associated with activation of the Wnt/β-catenin pathway, although direct target validation remains to be established.
NO LEVEL ASSIGNED: This journal requires that authors assign a level of evidence to each submission to which Evidence-Based Medicine rankings are applicable. This excludes Review Articles, Book Reviews, and manuscripts that concern Basic Science, Animal Studies, Cadaver Studies, and Experimental Studies. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .