Asian Pacific journal of cancer prevention : APJCP · 2015
S100A16 is a Prognostic Marker for Lung Adenocarcinomas.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод пока не подготовлен
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Saito Keita, Kobayashi Makoto, Nagashio Ryo, Ryuge Shinichiro, Katono Ken, Nakashima Hiroyasu, Tsuchiya Benio, Jiang Shi-Xu, Saegusa Makoto, Satoh Yukitoshi, Masuda Noriyuki, Sato Yuichi
Аннотация
BACKGROUND: Many functional molecules controlling diverse cellular function are included in low-molecular weight proteins and peptides.
MATERIALS AND METHODS: To identify proteins controlling function in lung adenocarcinomas (AC), we performed two-dimensional gel electrophoresis employing tricine-SDS polyacrylamide in the second dimension (tricine 2-DE). This system was able to detect proteins under 1 kDa even with post- translational modifications. To confirm the utility of detected proteins as novel tumor markers for AC, we performed immunohistochemical analysis using 170 formalin-fixed and paraffin-embedded lung AC tissues.
RESULTS: Tricine 2-DE revealed that five proteins including S100A16 were overexpressed in lung AC-derived cells compared with lung squamous cell carcinoma, small cell carcinoma, and large cell neuroendocrine carcinoma- derived cells. Immunohistochemically, S100A16 showed various subcellular localization in lung cancer tissues and a membranous staining status was correlated with the T-factor (P=0.0008), pathological stage (P=0.0015), differentiation extent (P=0.0001), lymphatic invasion (P=0.0007), vascular invasion (P=0.0001), pleural invasion (P=0.0087), and gender (P=0.039), but not with the age or smoking history. More importantly, membranous staining of S100A16 was significantly correlated with a poorer overall survival of either stage I (P=0.0088) or stage II / III (P=0.0003) lung AC patients, and multivariate analysis confirmed that membranous expression of S100A16 was an independent adverse prognostic indicator (P=0.0001).
CONCLUSIONS: The present results suggest that S100A16 protein is a novel prognostic marker for lung AC.
MATERIALS AND METHODS: To identify proteins controlling function in lung adenocarcinomas (AC), we performed two-dimensional gel electrophoresis employing tricine-SDS polyacrylamide in the second dimension (tricine 2-DE). This system was able to detect proteins under 1 kDa even with post- translational modifications. To confirm the utility of detected proteins as novel tumor markers for AC, we performed immunohistochemical analysis using 170 formalin-fixed and paraffin-embedded lung AC tissues.
RESULTS: Tricine 2-DE revealed that five proteins including S100A16 were overexpressed in lung AC-derived cells compared with lung squamous cell carcinoma, small cell carcinoma, and large cell neuroendocrine carcinoma- derived cells. Immunohistochemically, S100A16 showed various subcellular localization in lung cancer tissues and a membranous staining status was correlated with the T-factor (P=0.0008), pathological stage (P=0.0015), differentiation extent (P=0.0001), lymphatic invasion (P=0.0007), vascular invasion (P=0.0001), pleural invasion (P=0.0087), and gender (P=0.039), but not with the age or smoking history. More importantly, membranous staining of S100A16 was significantly correlated with a poorer overall survival of either stage I (P=0.0088) or stage II / III (P=0.0003) lung AC patients, and multivariate analysis confirmed that membranous expression of S100A16 was an independent adverse prognostic indicator (P=0.0001).
CONCLUSIONS: The present results suggest that S100A16 protein is a novel prognostic marker for lung AC.
Предметные рубрики MeSH
AdenocarcinomaAgedBiomarkers, TumorBlood VesselsCarcinoma, NeuroendocrineCarcinoma, Squamous CellCell Line, TumorCell MembraneFemaleHumansImmunohistochemistryLung NeoplasmsMaleMiddle AgedNeoplasm GradingNeoplasm InvasivenessNeoplasm StagingPleuraPrognosisS100 ProteinsSex FactorsSmall Cell Lung CarcinomaSurvival Rate