Zhen ci yan jiu = Acupuncture research · 2011
[Effect of electroacupuncture on serum proinflammatory cytokine levels and pancreatic nuclear factor kappa-b expression in acute pancreatitis rats].
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Xue Qi-ming, Ning Li, Xue Ping, Wang Cheng-wei, He Hong-bo
Аннотация
OBJECTIVE: To observe the effects of electroacupuncture (EA) of "Zusanli" (ST 36) on serum TNF-alpha and IL-6 contents and pancreatic nuclear factor kappa-B (NF-kappaB) expression in acute pancreatitis rats.
METHODS: Sixty-six male SD rats were randomly divided into sham operation (sham), model and EA groups (n=22). Acute pancreatitis model was established by intra-pancreatic duct injection of 3.5% sodium taurocholate (0.1 mL/100 g). EA (2 Hz /100 Hz, 2 mA) was applied to bilateral ST 36 for 30 min after modeling and at the end of the experiment. The animals were killed at 3 h (n=7), 6 h (n=7) and 12 h (n=8) after modeling. The expression of pancreatic NF-,cB P65 was detected by immunohistochemical staining, and serum TNF-alpha and IL-6 contentswere determined by ELISA. The pathological changes of pancreatic tissue were displayed by H. E. staining and the quantity of ascite was measured by electronic balance.
RESULTS: The pathologic score, ascite quantity, serum TNFalpha and IL-6 contents, and pancreatic NF-kappaB P 65 expression levels at 3 h, 6 h and 12 h after modeling were significantly higher in the model group than in the sham group (P < 0.05). Compared with the model group, the pathologic score, ascite quantity, serum TNF-alpha and IL-6 contents, and pancreatic NF-kappaB P 65 expression levels at 3 h, 6 h and 12 h were significantly decreased in the EA group (P < 0.05). Microscopic observation displayed that the necrosis of the pancreatic acinar cells and infiltration of inflammatory cells were lighter in the EA group than in the model group.
CONCLUSION: EA at ST 36 is able to down-regulate sodium taurocholate injection incduced increase of serum TNF-alpha and IL-6 contents, and pancreatic NF-kappaB P65 expression level in acute pancreatitis rats, which may contribute to its effect in relieving necrosis of the pancreatic acinar cells and infiltration of pancreatic inflammatory cells.
METHODS: Sixty-six male SD rats were randomly divided into sham operation (sham), model and EA groups (n=22). Acute pancreatitis model was established by intra-pancreatic duct injection of 3.5% sodium taurocholate (0.1 mL/100 g). EA (2 Hz /100 Hz, 2 mA) was applied to bilateral ST 36 for 30 min after modeling and at the end of the experiment. The animals were killed at 3 h (n=7), 6 h (n=7) and 12 h (n=8) after modeling. The expression of pancreatic NF-,cB P65 was detected by immunohistochemical staining, and serum TNF-alpha and IL-6 contentswere determined by ELISA. The pathological changes of pancreatic tissue were displayed by H. E. staining and the quantity of ascite was measured by electronic balance.
RESULTS: The pathologic score, ascite quantity, serum TNFalpha and IL-6 contents, and pancreatic NF-kappaB P 65 expression levels at 3 h, 6 h and 12 h after modeling were significantly higher in the model group than in the sham group (P < 0.05). Compared with the model group, the pathologic score, ascite quantity, serum TNF-alpha and IL-6 contents, and pancreatic NF-kappaB P 65 expression levels at 3 h, 6 h and 12 h were significantly decreased in the EA group (P < 0.05). Microscopic observation displayed that the necrosis of the pancreatic acinar cells and infiltration of inflammatory cells were lighter in the EA group than in the model group.
CONCLUSION: EA at ST 36 is able to down-regulate sodium taurocholate injection incduced increase of serum TNF-alpha and IL-6 contents, and pancreatic NF-kappaB P65 expression level in acute pancreatitis rats, which may contribute to its effect in relieving necrosis of the pancreatic acinar cells and infiltration of pancreatic inflammatory cells.