Fertility and sterility · 2008
Live birth after vitrification of in vitro matured human oocytes.
Клинический случайСтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод пока не подготовлен
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Chian Ri-Cheng, Gilbert Lucy, Huang Jack Y J, Demirtas Ezgi, Holzer Hananel, Benjamin Alice, Buckett William M, Tulandi Togas, Tan Seang Lin
Аннотация
OBJECTIVE: To report the first healthy live birth from immature oocytes retrieved in a natural menstrual cycle, followed by in vitro maturation (IVM) and cryopreservation of the oocytes by vitrification.
DESIGN: Case report.
SETTING: University-based tertiary medical center.
PATIENT(S): A 27-year-old woman with tubal disease and polycystic ovaries.
INTERVENTION(S): Immature oocytes were retrieved by transvaginal ultrasound guided follicle aspiration on day 13 of her natural menstrual cycle, matured in vitro and vitrified. The oocytes were thawed in a subsequent menstrual cycle, inseminated by intracytoplasmic sperm injection, and the resulting embryos transferred.
MAIN OUTCOME MEASURE(S): Oocyte maturation and survival rates, pregnancy, and live birth.
RESULT(S): One metaphase II and 18 germinal vesicle stage oocytes were collected; 16 out of 18 germinal vesicle oocytes matured, and a total of 17 oocytes were vitrified. After thawing, four IVM oocytes survived; three embryos were transferred. The woman went on to deliver a single healthy live baby at term.
CONCLUSION(S): We provide proof-of-principle evidence that the novel fertility preservation strategy of immature oocyte retrieval, IVM, and vitrification of oocytes can lead to successful pregnancy and healthy live birth.
DESIGN: Case report.
SETTING: University-based tertiary medical center.
PATIENT(S): A 27-year-old woman with tubal disease and polycystic ovaries.
INTERVENTION(S): Immature oocytes were retrieved by transvaginal ultrasound guided follicle aspiration on day 13 of her natural menstrual cycle, matured in vitro and vitrified. The oocytes were thawed in a subsequent menstrual cycle, inseminated by intracytoplasmic sperm injection, and the resulting embryos transferred.
MAIN OUTCOME MEASURE(S): Oocyte maturation and survival rates, pregnancy, and live birth.
RESULT(S): One metaphase II and 18 germinal vesicle stage oocytes were collected; 16 out of 18 germinal vesicle oocytes matured, and a total of 17 oocytes were vitrified. After thawing, four IVM oocytes survived; three embryos were transferred. The woman went on to deliver a single healthy live baby at term.
CONCLUSION(S): We provide proof-of-principle evidence that the novel fertility preservation strategy of immature oocyte retrieval, IVM, and vitrification of oocytes can lead to successful pregnancy and healthy live birth.